Genes and Proteins for the Biosynthesis of the Lantibiotic 107891

Abstract

The Invention relates to the field of lantibiotics, and more specifically to the isolation of nucleic acid molecules that code for the enzymes required for the biosynthetic pathway of the lantibiotic 107891 and the homologues thereof.

1 - 34 . (canceled) 
     
     
         35 . A recombinant DNA vector which comprises a nucleotide sequence encoding a polypeptide that, over its entire length, is at least 65% identical in amino acid sequence to a polypeptide encoded by SEQ ID NO: 7. 
     
     
         36 . A host cell transformed with the vector of  claim 35 . 
     
     
         37 . The transformed host cell of  claim 36  which belongs to the order Actinomycetales. 
     
     
         38 . The transformed host cell of  claim 37  which belongs to the family selected from the group consisting of Streptosporangiaceae, Micromonosporaceae, Pseudonocardiaceae and Streptomycetaceae. 
     
     
         39 . The transformed host cell according to  claim 38  which belongs to the genera selected from the group consisting of  Microbispora, Actinoplanes, Planomonospora , and  Streptomyces.    
     
     
         40 . The transformed host cell of  claim 36  which belongs to the order Bacillales. 
     
     
         41 . The transformed host cell of  claim 37  which belongs to the family selected from the group consisting of Bacillaceae, Lactobacillaceae, Streptococcaceae and Staphylococcaccae. 
     
     
         42 . The transformed host cell of  claim 38  which belongs to the genera selected from the group consisting of  Bacillus, Lactococcus, Streptococcus , and  Staphylococcus.    
     
     
         43 . The transformed host cell of  claim 36  which belongs to the species  Escherichia coli.    
     
     
         44 . A method for increasing production of 107891 by a microorganism capable of producing 107891, said method comprising: transforming with a recombinant DNA vector of  claim 35  a microorganism that produces 107891, wherein said DNA vector is an expression vector. 
     
     
         45 . A recombinant DNA vector which comprises a nucleotide sequence encoding a polypeptide that, over its entire length, is at least 65% identical in amino acid sequence to a polypeptide encoded by SEQ ID NO: 8. 
     
     
         46 . A host cell transformed with the vector of  claim 45 . 
     
     
         47 . The transformed host cell of  claim 46  which belongs to the order Actinomycetales. 
     
     
         48 . The transformed host cell of  claim 47  which belongs to the family selected from the group consisting of Streptosporangiaceae, Micromonosporaceae, Pseudonocardiaceae and Streptomycetaceae. 
     
     
         49 . The transformed host cell according to  claim 48  which belongs to the genera selected from the group consisting of  Microbispora, Actinoplanes, Planomonospora , and  Streptomyces.    
     
     
         50 . The transformed host cell of  claim 46  which belongs to the order Bacillales. 
     
     
         51 . The transformed host cell of  claim 47  which belongs to the family selected from the group consisting of Bacillaceae, Lactobacillaceae, Streptococcaceae and Staphylococcaccae. 
     
     
         52 . The transformed host cell of  claim 48  which belongs to the genera selected from the group consisting of  Bacillus, Lactococcus, Streptococcus , and  Staphylococcus.    
     
     
         53 . The transformed host cell of  claim 46  which belongs to the species  Escherichia coli.    
     
     
         54 . A method for increasing production of 107891 by a microorganism capable of producing 107891, said method comprising: transforming with a recombinant DNA vector of  claim 45  a microorganism that produces 107891, wherein said DNA vector is an expression vector.