Specific sorbent for binding proteins and peptides, and separation method using the same

Abstract

Sorbent comprising a solid support material, the surface of which comprises first residues comprising a binuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S, and second residues comprising a mononuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S.

1 - 16 . (canceled) 
     
     
         17 . A sorbent comprising a solid support material, wherein the surface of the sorbent comprises
 i) a first residue comprising a binuclear heteroaromatic structure comprising carbon atoms and at least one heteroatom selected from N, O, and S; and   ii) a second residue comprising a mononuclear heteroaromatic structure comprising carbon atoms and at least one heteroatom selected from N, O, and S;   
       wherein the first and second residues are not directly connected with each other, and wherein the first and second residues are separately attached to the bulk solid support material or a polymer film supported by the solid support material as a carrier. 
     
     
         18 . The sorbent of  claim 17 , wherein the binuclear heteroaromatic structure is a benzopyrrole (indole) structure selected from aza-benzopyrrole structures, oxa-benzopyrrole structures and thia-benzopyrrole structures. 
     
     
         19 . The sorbent of  claim 17 , wherein the binuclear heteroaromatic structure is a benzopyridine (quinoline or isoquinoline) structure selected from aza-benzopyridine structures, oxa-benzopyridine structures and thia-benzopyridine structures. 
     
     
         20 . The sorbent of  claim 17 , wherein the mononuclear heteroaromatic structure is a pyrrole structure selected from aza-pyrrole structures, oxa-pyrrole structures, thia-pyrrole structures and 3-azapyrrole (imidazole). 
     
     
         21 . The sorbent of  claim 17 , wherein the first and/or second residue comprise(s) a covalently bound and conformationally flexible linker of a length of from 1 to 20 atoms. 
     
     
         22 . The sorbent of  claim 17 , wherein the surface of the solid support material additionally comprises a third residue, and optionally, a fourth residue. 
     
     
         23 . The sorbent of  claim 22 , wherein the third residue comprises an amine structure, an amide structure or a primary amine structure. 
     
     
         24 . The sorbent of  claim 22 , wherein the first, second, and third residue are present in a molar ratio of about 1:1:2. 
     
     
         25 . The sorbent of  claim 17 , wherein the surface of the solid support material is covered with a film of a polymer comprising a first and a second functional group, wherein the first and a second functional group may be the same or may be different from each other, and wherein the polymer is a carrier for the first and second residues, and optionally, a third and a fourth residue. 
     
     
         26 . The sorbent of  claim 25 , wherein the polymer exhibits individual chains which are covalently crosslinked with each other, and wherein the polymer is not covalently bound to the surface of the solid support material. 
     
     
         27 . The sorbent of  claim 25 , wherein the polymer is a polyamine, a polyvinyl amine, a copolymer or a polymer blend comprising a polyamine. 
     
     
         28 . The sorbent of  claim 25 , wherein a first portion of the first and second functional groups of the polymer is crosslinked with at least one crosslinking reagent, and wherein a second portion of the first and second functional groups of the crosslinked polymer is bound to the first residue, the second residue, and, optionally, a third and a fourth residue. 
     
     
         29 . A method for preparing the sorbent of  claim 28 , comprising:
 (i) providing a polymer having a first and a second functional group;   (ii) adsorbing a film of the polymer onto a surface of a solid support material;   (iii) crosslinking a first portion of the functional groups of the adsorbed polymer with at least one crosslinking reagent; and   (iv) binding a second portion of the functional groups of the crosslinked polymer with the first residue, the second residue, and, optionally, the third and forth residues.   
     
     
         30 . A method of separating or increasing the concentration and/or purity of a protein or a peptide from a mixture containing the protein or the peptide, comprising:
 (i) contacting the mixture which is dissolved or suspended in a first liquid with the sorbent of  claim 17  for a period of time sufficient to bind the protein or peptide to the sorbent;   (ii) optionally, rinsing the sorbent with a second liquid;   (iii) contacting the sorbent with a third liquid for a period of time sufficient to release the bound protein or peptide from the sorbent;   (iv) separating the liquid from the sorbent to obtain the protein or peptide; and   (v) optionally, washing and/or regenerating the sorbent with a fourth and/or fifth liquid.   
     
     
         31 . The method of  claim 30 , wherein the pH of the first liquid and the second liquid is close to the isoelectric point of the protein or peptide. 
     
     
         32 . The method of  claim 30 , wherein the pH of the first liquid is in a range of from 5.5 to 8.5 and the pH of the third liquid is in a range of from 3 to 6.5. 
     
     
         33 . The method of  claim 30 , wherein the protein or peptide has an isoelectric point of from 5.5 to 8.5 and a molecular weight of from 100 to 500,000 Da.