Therapeutic composition produced using chinese gall and hydrogen peroxide

Abstract

Methods of and compositions for producing and using plant-based materials are provided. The methods include using biopolymers or their synthetic equivalents combined with a stable source of reactive oxygen species that when applied to or combined with a separate source of oxido-reducing enzyme or catalyst will cause the formation of an activated biopolymer with increased protein binding affinity and microbial control activities.

We claim: 
     
         1 . An anti-bacterial composition prepared by:
 a) combining Chinese gall with water and heating at 80° C. to 150° C. to substantially denature enzymes endogenous to said pomegranate rind and   b) adding from 1% to less than 10% of hydrogen peroxide to the product obtained from part (a).   
     
     
         2 . The anti-bacterial composition of  claim 1 , wherein the composition endogenously contains a component selected from the group consisting of a tannin, a lignin, a flavonoid, a hydroxycoumarin and an alkaloid. 
     
     
         3 . The anti-bacterial composition of  claim 1 , wherein said composition is in a dose ranging from 5 μg to 2500 μg. 
     
     
         4 . The anti-bacterial composition of  claim 1 , wherein the heating in part (a) is carried out from 80° C. to 110° C. 
     
     
         5 . The anti-bacterial composition of  claim 1 , wherein 1% to 2% of hydrogen peroxide is added to part (b). 
     
     
         6 . The anti-bacterial composition of  claim 1 , wherein the process further comprises diluting to a hydrogen peroxide concentration of 25 ppm. 
     
     
         7 . A kit comprising a dry form of the anti-bacterial composition of  claim 1 . 
     
     
         8 . A method for treating a bacterial infection, comprising administering to a subject in need thereof, an effective amount of the anti-bacterial composition of  claim 1 . 
     
     
         9 . The method of  claim 8 , wherein the administering comprises administering orally in a dose ranging from 5.0 μg to 2500 μg per day based on dry weight equivalent. 
     
     
         10 . A method of treating bacteria-infected water, comprising administering to said water an effective amount of the anti-bacterial composition of  claim 1 . 
     
     
         11 . A method of treating or preventing a bacterial infection in a wounded tissue, comprising administering to said tissue, an effective amount of the anti-bacterial composition of  claim 1 . 
     
     
         12 . A method for producing an anti-bacterial composition, wherein the method comprises;
 a) combining Chinese gall with water and heating at 80° C. to 150° C. to substantially denature enzymes endogenous to said pomegranate rind and   b) adding from 1% to less than 10% of hydrogen peroxide to the product obtained from part (a).   
     
     
         13 . The method of  claim 12 , wherein said heating in part (a) is carried out from 80° C. to 110° C. 
     
     
         14 . The method of  claim 12 , wherein 1% to 2% of hydrogen peroxide is added in part (b). 
     
     
         15 . The method of  claim 12 , The anti-bacterial composition of  claim 1 , further comprising diluting to a hydrogen peroxide concentration of 25 ppm. 
     
     
         16 . An anti-bacterial composition prepared by:
 a) obtaining a water soluble extract of Chinese gall having at least substantially no active endogeneous enzymes;   b) combining the water soluble extract from part (a) with water to create a solution;   c) adding hydrogen peroxide to the solution from part (b) at a concentration of less than 10%; and,   
     
     
         17 . The anti-bacterial composition of  claim 16 , further comprising diluting the solution to a hydrogen peroxide concentration of 25 ppm. 
     
     
         18 . A dosage form of an anti-bacterial solution comprising at least 5 ug of a water soluble extract of Chinese gall and at least 25 ppm hydrogen peroxide. 
     
     
         19 . The dosage form of  claim 18  in the form of an aqueous solution. 
     
     
         20 . The dosage form of  claim 18  in a dry form.