Attenuated Live Vaccines for Aquatic Animals

Abstract

Safe and effective live vaccines against bacteria infecting aquatic animals were created through the induction of novobiocin-resistance in liquid culture and novobiocin- and rifampicin-resistance in liquid culture.

1 - 34 . (canceled) 
     
     
         35 . An isolated attenuated strain of  Aeromonas hydrophila  deposited with USDA ARS Patent Culture Collection, selected from the group consisting of accession number NRRL B-50369, accession number NRRL B-50418, accession number NRRL B-50419, and mixtures thereof. 
     
     
         36 . A vaccine composition for protecting aquatic animals against infection by  Aeromonas hydrophila  comprising:
 a. an immunologically effective amount of an isolated attenuated strain of  Aeromonas hydrophila  resistant to novobiocin or a novobiocin-like antibiotic and deposited with USDA ARS Patent Culture Collection, selected from the group consisting of accession number NRRL B-50369, accession number NRRL B-50418, accession number NRRL B-50419, and mixtures thereof; and   b. a carrier.   
     
     
         37 . A method of protecting aquatic animals against infection by  Aeromonas hydrophila  comprising administering to said aquatic animals the isolated attenuated strain of  Aeromonas hydrophila  of  claim 35 . 
     
     
         38 . A method of protecting aquatic animals against infection by  Aeromonas hydrophila  comprising administering to said aquatic animals the vaccine composition of  claim 36 .