Omega-9 quality brassica juncea

Abstract

The invention relates to improved Brassica species, including Brassica juncea, improved oil and meal from Brassica juncea, methods for generation of such improved Brassica species, and methods for selection of Brassica lines. Further embodiments relate to seeds of Brassica juncea comprising an endogenous oil having increased oleic acid content and decreased linolenic acid content relative to presently existing commercial cultivars of Brassica juncea, seeds of Brassica juncea having traits for increased oleic acid content and decreased linolenic acid content in seed oil stably incorporated therein, and one or more generations of progeny plants produced from said seeds.

1 . A  Brassica juncea  seed having an endogenous fatty acid content comprising at least 70.0% oleic acid by weight and less than 5.0% linolenic acid by weight. 
     
     
         2 . The  Brassica juncea  seed of  claim 1  whose seeds have an endogenous fatty acid content comprising 70.0% to 85.0% oleic acid by weight. 
     
     
         3 . The seed according to  claim 1 , wherein the seed has total extractable oils comprising a fatty acid content of at least 70.0% oleic acid by weight and less than 5.0% linolenic acid by weight. 
     
     
         4 . The  Brassica juncea  seed of  claim 1 , containing a mutated fad2 gene. 
     
     
         5 . The  Brassica juncea  seed of  claim 1 , containing a mutated fad3 gene. 
     
     
         6 . A method of producing a  Brassica juncea  seeds have an endogenous fatty acid content comprising at least 70.0% oleic acid by weight and less than 5.0% linolenic acid by weight, comprising introducing into a  Brassica juncea  plant, through traditional crossing methods, one or more nucleic acid sequences selected from the group consisting of a mutated fad2a nucleic acid sequence, a mutated fad2b nucleic acid sequence, a mutated fad3a nucleic acid sequence, and a mutated fad3b nucleic acid sequence. 
     
     
         7 .  Brassica juncea  seeds, said seeds having one or more non-recombinant fad2 and fad3 genes from one or more genomic components of a  Brassica  aa and/or  Brassica  bb genome, said seeds having a fatty acid content comprising at least 70.0% oleic acid by weight and less than 5.0% linolenic acid by weight. 
     
     
         8 . One or more generations of progeny plants produced from at least one of the seeds of  claim 7 , wherein seeds of said progeny plants comprise at least 70.0% oleic acid by weight and less than 5.0% linolenic acid by weight. 
     
     
         9 . A crop of  Brassica juncea  producing seeds having an endogenous oil content which averages, across the crop, at least 70.0% oleic acid by weight and less than 5.0% linolenic acid by weight. 
     
     
         10 . One or more generations of progeny crops resulting from at least one of the seeds of the crop of  claim 9 . 
     
     
         11 . Seeds of a  Brassica juncea  cultivar having, on average, an endogenous oil content which averages, across the crop, at least 70.0% oleic acid by weight and less than 5.0% linolenic acid by weight. 
     
     
         12 . An oilseed plant produced by crossing the  Brassica juncea  cultivar of  claim 11  with a plant of a  Brassica  species. 
     
     
         13 . Meal from the seeds of  claim 11 . 
     
     
         14 . The meal of  claim 13 , wherein the meal is in the form of crushed seeds, press cake, white flake, or the meal from conventional crushing and solvent extraction processes.