Biopsy support with sectionable resilient cellular material

Abstract

A histologic tissue sample support device includes a tissue support formed of material that can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue. A resilient cellular material is coupled to the tissue support and is configured to engage and retain tissue in place during processing and embedding. The resilient cellular material is also capable of successful sectioning in the microtome and porous to allow infiltration of the solvents and chemicals used to fix, process and stain tissue, and of embedding material used to embed the tissue while the tissue is retained by the resilient cellular material.

What is claimed is: 
     
         1 . A method for preparing one or more biopsy tissue samples for histological examination, comprising:
 positioning a tissue sample in close proximity to a microtome sectionable support;   immobilizing the tissue sample on a the support by contacting the tissue sample with a microtome sectionable, resilient cellular material coupled to the tissue support;   subjecting the microtome sectionable support, resilient cellular material and the tissue sample to a process that replaces fluid in the tissue sample with a hardenable material;   embedding the microtome sectionable support, resilient cellular material and the tissue sample in an embedding material;   hardening the embedding material into a block; and   slicing the block with a microtome into thin slices of the embedding material, the microtome sectionable support, the resilient cellular material and the tissue sample.   
     
     
         2 . The method of  claim 1 , wherein the embedding material is wax. 
     
     
         3 . The method of  claim 1 , wherein the microtome sectionable support further comprises a bottom portion configured to hold the tissue sample and a lid holding the resilient cellular material, and the step of immobilizing the tissue sample further comprises:
 closing the lid on top of the tissue sample to trap the tissue sample between the resilient cellular material and the bottom portion.   
     
     
         4 . The method of  claim 3 , wherein the bottom portion includes an interior space surrounded by at least one side wall and the positioning and immobilizing steps further comprise:
 placing the tissue sample within the interior space; and   inserting the resilient cellular material at least partially into the interior space and into contact with the tissue sample.   
     
     
         5 . The method of  claim 3 , wherein the resilient cellular material deforms during the immobilizing step to create a three dimensional space that receives the tissue sample. 
     
     
         6 . The method of  claim 1 , wherein the microtome sectionable support is coupled to a frame prior to being subjected to the process for replacing fluid in the tissue sample with the hardenable material, and the method further comprises securing the frame in the microtome prior to slicing the block. 
     
     
         7 . The method of  claim 6 , wherein prior to embedding the microtome sectionable support, resilient cellular material and the tissue sample in the embedding material, the microtome sectionable support is moved from a first position within the frame to a second position in which the support, resilient cellular material and tissue sample are exposed for simultaneous sectioning in the microtome.