US2014141457A1PendingUtilityA1

DETECTION KIT CONTAINING A NOVEL RECOMBINANT 15-kDA POLYPEPTIDE USEFUL FOR DETECTING HUMAN INFECTION WITH BARTONELLA HENSELAE

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Assignee: HOEY JOHN GPriority: Aug 22, 2008Filed: Oct 8, 2013Published: May 22, 2014
Est. expiryAug 22, 2028(~2.1 yrs left)· nominal 20-yr term from priority
Inventors:John G. HoeyLisa P. HuangMartin E. AdelsonEli Mordechai
G01N 2469/20G01N 33/56911C07K 14/195G01N 33/6893
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Claims

Abstract

Disclosed are the cloning and expression of a novel antigen of Bartonella henselae . The recombinant polypeptide is found to be highly immunogenic and is useful as a diagnostic test antigen. The polypeptide of the present invention provides the basis of a diagnostic assay that is sensitive, rapid and accurate diagnosis of infection with Bartonella henselae using patient's sera. Disclosed also are the ELISA for both IgG and IgM and allows diagnosis of early and late infection.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . A composition for detection of the presence of anti-15-kDa IgG or IgM antibodies in a human suspected of  Bartonella henselae  infection, comprising:
 a) an isolated recombinant 15-kDa polypeptide, said isolated recombinant 15-kDa polypeptide having an amino acid sequence of SEQ ID NO: 2; and   b) a support, said support having said isolated recombinant 15-kDa polypeptide immobilized thereon,   wherein said composition detects the presence of anti-15-kDa IgG or IgM antibodies in a human suspected of  Bartonella henselae  infection.   
     
     
         2 . The composition of  claim 1 , wherein said support is selected from the group consisting of polyethylene, polypropylene and glass. 
     
     
         3 . The composition of  claim 2 , wherein said support is polypropylene. 
     
     
         4 . The composition of  claim 1 , wherein said support is a microtiter well. 
     
     
         5 . The composition of  claim 1 , further comprising:
 c) packaging material.   
     
     
         6 . The composition of  claim 5 , wherein said packaging material comprises an instruction for detecting the presence of an antibody against  Bartonella henselae.    
     
     
         7 . The composition of  claim 1 , further comprising:
 d) a vector and a promoter.   
     
     
         8 . The composition of  claim 7 , wherein said vector is selected from the group consisting of pET, pENTR and pCR 8/GW/TOPO and said promoter is selected from the group consisting of lac promoter, trp promoter and tac promoter. 
     
     
         9 . The composition of  claim 8 , wherein said vector is pET and said promoter is lac promoter.

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