US2014147842A1PendingUtilityA1

Method for detecting single nucleotide polymorphisms

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Assignee: YOTANI TAKUYAPriority: Jan 12, 2011Filed: Jan 12, 2012Published: May 29, 2014
Est. expiryJan 12, 2031(~4.5 yrs left)· nominal 20-yr term from priority
Inventors:Takuya YotaniEiji KiyotohKoji Ushizawa
C12Q 1/6844C12Q 1/6858G01N 30/96C12Q 2565/137C12Q 1/6827
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Claims

Abstract

An object of the present invention is to provide a method for rapidly and simply detecting single nucleotide polymorphisms. The present invention is a method for detecting single nucleotide polymorphisms, comprising analyzing wild-type and mutant-type products amplified by an AS-PCR method using ion-exchange chromatography.

Claims

exact text as granted — not AI-modified
1 . A method for detecting single nucleotide polymorphisms, comprising analyzing wild-type and mutant-type products amplified by an AS-PCR method using ion-exchange chromatography. 
     
     
         2 . The method for detecting single nucleotide polymorphisms according to  claim 1 , wherein the size of the products amplified by an AS-PCR method is 200 bp or less and the difference in the chain length between the wild-type and the mutant-type is 10 bp or less. 
     
     
         3 . The method for detecting single nucleotide polymorphisms according to  claim 1 , comprising using an eluent containing a guanidine salt derived from guanidine represented by formula (1) below in ion-exchange chromatography: 
       
         
           
           
               
               
           
         
       
     
     
         4 . The method for detecting single nucleotide polymorphisms according to  claim 3 , wherein the guanidine salt is guanidine hydrochloride or guanidine sulfate. 
     
     
         5 . The method for detecting single nucleotide polymorphisms according to  claim 2 , comprising using an eluent containing a guanidine salt derived from guanidine represented by formula (1) below in ion-exchange chromatography: 
       
         
           
           
               
               
           
         
       
     
     
         6 . The method for detecting single nucleotide polymorphisms according to  claim 5 , wherein the guanidine salt is guanidine hydrochloride or guanidine sulfate.

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