Factor IX: Remodeling and Glycoconjugation of Factor IX

Abstract

The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.

1 - 38 . (canceled) 
     
     
         39 . A covalent conjugate between a Factor IX peptide and a poly(ethylene glycol) that alters a property of said peptide, wherein said poly(ethylene glycol) is covalently attached to said Factor IX peptide at a preselected glycosyl or amino acid residue of said Factor IX peptide via an intact glycosyl linking group. 
     
     
         40 . (canceled) 
     
     
         41 . The covalent conjugate of  claim 39 , wherein said poly(ethylene glycol) and an intact glycosyl linking group precursor are linked as a covalently attached unit to said Factor IX peptide via the action of an enzyme, said enzyme converting said precursor to said intact glycosyl linking group, thereby forming said conjugate. 
     
     
         42 . The covalent conjugate of  claim 39  comprising:
 a first poly(ethylene glycol) covalently linked to a first glycosyl residue of said Factor IX peptide via a first intact glycosyl linking group, and 
 a second poly(ethylene glycol) covalently linked to a second glycosyl residue of said Factor IX peptide via a second intact glycosyl linking group. 
 
     
     
         43 . The covalent conjugate of  claim 42 , wherein said first glycosyl residue and said second glycosyl residue are structurally identical. 
     
     
         44 . The covalent conjugate of  claim 42 , wherein said first glycosyl residue and said second glycosyl residue have different structures. 
     
     
         45 - 46 . (canceled) 
     
     
         47 . The covalent conjugate of  claim 39 , wherein said Factor IX peptide is remodeled prior to forming said conjugate. 
     
     
         48 . The covalent conjugate of  claim 47 , wherein the remodeled Factor IX peptide is remodeled to introduce an acceptor moiety for said intact glycosyl linking group. 
     
     
         49 - 51 . (canceled) 
     
     
         52 . The covalent conjugate of  claim 39 , wherein said poly(ethylene glycol) has a molecular weight distribution that is essentially homodisperse. 
     
     
         53 - 112 . (canceled)