Test apparatus and target measurement method using the same

Abstract

A test apparatus and method for measuring a concentration of a target by correcting for an impact of hemoglobin are provided. The target measurement method includes measuring an absorbance of hemoglobin in a sample, measuring an absorbance of a target in the sample, determining variation of the absorbance of the target according to the measured absorbance of the hemoglobin, and correcting the absorbance of the target by subtracting the determined variation of the absorbance of the target from the measured absorbance of the target.

What is claimed is: 
     
         1 . A target measurement method comprising:
 measuring an absorbance of hemoglobin in a sample;   measuring an absorbance of a target in the sample;   determining a variation of the absorbance of the target according to the measured absorbance of the hemoglobin; and   correcting the absorbance of the target by subtracting the determined variation of the absorbance of the target from the measured absorbance of the target.   
     
     
         2 . The target measurement method according to  claim 1 , wherein the measuring the absorbance of hemoglobin comprises:
 irradiating the sample with light to measure absorbance of the sample; and   determining the absorbance of hemoglobin in the sample based on the measured absorbance of the sample and a pre-determined equation for calculation of the absorbance of the hemoglobin.   
     
     
         3 . The target measurement method according to  claim 2 , wherein the irradiating the sample comprises irradiating a sample accommodated in a chamber of a reactor, excluding a reagent for detection of the target, with light having at least one predetermined wavelength. 
     
     
         4 . The target measurement method according to  claim 3 , wherein the reactor comprises:
 at least one reaction chamber accommodating the reagent for detection of at least one target in the sample; and   a control chamber excluding the reagent.   
     
     
         5 . The target measurement method according to  claim 2 , wherein the determining the absorbance of the hemoglobin comprises determining the absorbance of the hemoglobin based on the pre-determined equation for calculation of the absorbance of the hemoglobin independently from change in concentration of the target, when measurement results of the absorbance of the sample are input. 
     
     
         6 . The target measurement method according to  claim 4 , wherein the equation comprises a variable to be substituted with absorbance obtained by irradiating the sample accommodated in the control chamber with light having at least one predetermined wavelength and a coefficient for calculation of the absorbance of the hemoglobin independently from change in concentration of the target. 
     
     
         7 . The target measurement method according to  claim 1 , wherein the measuring the absorbance of the target comprises measuring the absorbance of the target in the sample by irradiating the sample accommodated in a chamber of a reactor, containing a reagent for detection of the target, with light having at least one predetermined wavelength. 
     
     
         8 . The target measurement method according to  claim 1 , wherein the determining the variation of the absorbance of the target comprises determining the variation of the absorbance of the target according to the measured absorbance of the hemoglobin based on a pre-determined correlation showing the variation of the absorbance of the target according to the absorbance of the hemoglobin. 
     
     
         9 . The target measurement method according to  claim 1 , further comprising determining a concentration of the target based on the corrected absorbance of the target after the absorbance of the target is corrected. 
     
     
         10 . The target measurement method according to  claim 1 , wherein the sample comprises blood plasma or serum. 
     
     
         11 . A test apparatus comprising:
 a light detection module configured to irradiate a sample with light and detect the light passed through the sample; and   a controller configured to measure an absorbance of hemoglobin in the sample and an absorbance of a target in the sample based on detection results of the light detection module, determine a variation of the absorbance of the target according to the measured absorbance of the hemoglobin, and correct the absorbance of the target by subtracting the determined variation of the absorbance of the target from the measured absorbance of the target.   
     
     
         12 . The test apparatus according to  claim 11 , wherein the controller is configured to control the light detection module to irradiate a sample accommodated in a chamber of a reactor, excluding a reagent for detection of the target, with light having at least one predetermined wavelength, and determine the absorbance of the hemoglobin independently from change in concentration of the target, based on a pre-determined equation for calculation of the absorbance of the hemoglobin, when the light detection module detects the light. 
     
     
         13 . The test apparatus according to  claim 12 , wherein the reactor comprises at least one reaction chamber configured to accommodate a reagent for detection of at least one target in the sample; and
 a control chamber excluding the reagent.   
     
     
         14 . The test apparatus according to  claim 13 , wherein the equation comprises a variable to be substituted with absorbance obtained by irradiating the sample accommodated in the control chamber with light having at least one predetermined wavelength and a coefficient for calculation of the absorbance of the hemoglobin independently from change in concentration of the target. 
     
     
         15 . The test apparatus according to  claim 11 , wherein the controller is configured to control the light detection module to irradiate a sample accommodated in a chamber of a reactor, containing a reagent for detection of the target, with light having at least one predetermined wavelength, and measure the absorbance of the target in the sample based on detection results when the light detection module detects the light. 
     
     
         16 . The test apparatus according to  claim 11 , wherein the controller is configured to measure the variation of the absorbance of the target according to the measured absorbance of the hemoglobin based on a pre-determined correlation showing the variation of the absorbance of the target according to the absorbance of the hemoglobin. 
     
     
         17 . The test apparatus according to  claim 16 , wherein the correlation is determined based on measurement results of absorbance of a target in each of a plurality of samples having the same concentration of the target and different concentrations of the hemoglobin. 
     
     
         18 . The test apparatus according to  claim 11 , wherein the controller is configured to determine a concentration of the target based on the corrected absorbance of the target when the absorbance of the target is corrected.