Polynucleotides and Polypeptides in Plants

Abstract

The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.

What is claimed is: 
     
         1 . A transgenic plant transformed with a DNA construct encoding a polypeptide having an amino acid sequence identity with a sequence selected from the group consisting of SEQ ID NO: 2N, wherein N=1-480, SEQ ID NO: 2N−1, where N=857-970, or SEQ ID NO: 989, 990, 991, 1001, 1002, 1012, 1018, 1021, 1022, 1025, 1027, 1028, 1029, 1034, 1050, 1051, 1072, 1073, 1074, 1075, 1076, 1091, 1092, 1093, 1094, 1095, 1109, 1110, 1111, 1112, 1150, 1165, 1166, 1167, 1168, 1169, 1189, 1190, 1191, 1197, 1198, 1199, 1213, 1214, 1215, 1216, 1226, 1227, 1233, 1239, 1246, 1247, 1258, 1259, 1269, 1307, 1308, 1309, 1310, 1323, 1329, 1330, 1331, 1332, 1338, 1339, 1340, 1361, 1362, 1373, 1374, 1375, 1384, 1389, 1390, 1391, 1396, 1411, 1412, 1413, 1414, 1424, 1435, 1436, 1437, 1448, 1456, 1457, 1458, 1459, 1460, 1472, 1483, 1484, 1500, 1508, 1510, 1511, 1520, 1538, 1539, 1540, 1541, 1542, 1543, 1563, 1564, 1565, 1566, 1567, 1568, 1569, 1582, 1583, 1594, 1611, 1612, 1618, 1619, 1620, 1626, 1627, 1635, 1636, 1640, 1641, 1655, 1656, 1657, 1658, 1672, 1673, 1680, 1682, 1686, 1687, 1688, 1689, 1696, 1702, 1703, 1945, 1947, 1949, 1951, 1953, 1955, 1957, 1959, 1961, 1963, 1965, 1967, 1969, 1971, and 1973;
 wherein the amino acid identity is selected from the group consisting of at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, or at least 86%, at least 87%, at least 88%, at least 90%, at least 95%, at least 98%, or 100% amino acid sequence identity; 
 wherein when the polypeptide is expressed in the transgenic plant, the polypeptide confers to the trangenic plant an enhanced trait, as compared to a control plant, selected from the group consisting of: 
 smaller size, smaller petals, smaller sepals, increased tolerance to  Erysiphe , increased resistance to  Erysiphe , increased tolerance to  Botrytis , increased tolerance to  Sclerotinia , increased tolerance to  Pseudomonas , increased tolerance to salt, increased tolerance to sucrose, increased tolerance to glucose, increased tolerance to cold, increased tolerance in heat, increased tolerance to osmotic stress, increased tolerance to drought, increased tolerance to nutrient-limiting conditions, increased tolerance to nitrogen-limiting conditions, increased tolerance to phosphate-free medium, increased tolerance to potassium-free medium, increased tolerance to freezing, altered C:N sensing, reduced sensitivity to ABA, insensitive to ABA, decreased seed oil content, increased seed protein content, increased seed protein content, decreased seed protein, late flowering, early flowering, sterile, less pollen production, embryo lethal, delayed senescence, premature senescence, fewer trichomes at seedling stage, altered leaf shape, curled leaves, serrated leaves, shiny leaves, dark green leaves, dark green plant, light green coloration, reduced seed color, greater size, increased seedling size, large leaves, larger seeds, greater biomass, increased root hairs, homeotic transformation, terminal flowers, short stamen filaments, abnormal anther development, reduced fertility, glabrous, altered trichome distribution, increased trichome size, increased trichome density, reduced branching, more vascular bundles in stem, loss of apical dominance, long petioles, upturned leaves, smaller petals, smaller sepals, reduced or absent petals, sepals and stamens, reduced petal abscission, leaf cell expansion, slow growth, photomorphogenesis in dark conditions, lethal when overexpressed, increased leaf unsaturated fatty acids, increased leaf chlorophyll, increased leaf carotenoids, increased anthocyanins in leaf, increased anthocyanins in root, increased anthocyanins in seed, reduced lignin, increased lutein content, increased seed lutein, decreased seed lutein, decreased leaf lutein, increased xanthophyll, increased leaf xanthophyll, increased arabinose, increased leaf arabinose, increased mannose, increased δ- and γ-tocopherol, increased leaf insoluble sugars, decreased insoluble sugars, increase in xylose, increased leaf rhamnose, increased fucose, decreased rhamnose, increased leaf fatty acids, increase in glucosinolate M39480, increased glucosinolate M39481, increase in 18:1 fatty acids, increased seed 16:0 fatty acid, increased seed 16:1 fatty acid, increased seed 18:0 fatty acid, increased seed 18:1 fatty acid, increased seed 18:3 fatty acid, increased seed 20:0 fatty acid, reduction in 16:3 fatty acid, reduction in 18:2 fatty acids, reduction in 20:1 fatty acids, reduction in 22:1 fatty acids, increased wax in leaves, and, decreased seed fatty acid composition. 
 
     
     
         2 . The transgenic plant of  claim 1 , wherein the transgenic plant is dicotyledenous. 
     
     
         3 . The transgenic plant of  claim 1 , wherein the transgenic plant is monocotyledenous. 
     
     
         4 . The transgenic plant of  claim 1 , wherein expression of the polypeptide is regulated by a constitutive, inducible, or tissue-specific promoter. 
     
     
         5 . A method for producing a transgenic plant having an enhanced trait as compared to a control plant, the method steps comprising:
 (a) transforming a target plant with a DNA construct encoding a polypeptide having an amino acid sequence identity with a sequence selected from the group consisting of SEQ ID NO: 2N, wherein N=1-480, SEQ ID NO: 2N−1, where N=857-970, or SEQ ID NO: 989, 990, 991, 1001, 1002, 1012, 1018, 1021, 1022, 1025, 1027, 1028, 1029, 1034, 1050, 1051, 1072, 1073, 1074, 1075, 1076, 1091, 1092, 1093, 1094, 1095, 1109, 1110, 1111, 1112, 1150, 1165, 1166, 1167, 1168, 1169, 1189, 1190, 1191, 1197, 1198, 1199, 1213, 1214, 1215, 1216, 1226, 1227, 1233, 1239, 1246, 1247, 1258, 1259, 1269, 1307, 1308, 1309, 1310, 1323, 1329, 1330, 1331, 1332, 1338, 1339, 1340, 1361, 1362, 1373, 1374, 1375, 1384, 1389, 1390, 1391, 1396, 1411, 1412, 1413, 1414, 1424, 1435, 1436, 1437, 1448, 1456, 1457, 1458, 1459, 1460, 1472, 1483, 1484, 1500, 1508, 1510, 1511, 1520, 1538, 1539, 1540, 1541, 1542, 1543, 1563, 1564, 1565, 1566, 1567, 1568, 1569, 1582, 1583, 1594, 1611, 1612, 1618, 1619, 1620, 1626, 1627, 1635, 1636, 1640, 1641, 1655, 1656, 1657, 1658, 1672, 1673, 1680, 1682, 1686, 1687, 1688, 1689, 1696, 1702, 1703, 1945, 1947, 1949, 1951, 1953, 1955, 1957, 1959, 1961, 1963, 1965, 1967, 1969, 1971, and 1973;   wherein the amino acid identity is selected from the group consisting of at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, or at least 86%, at least 87%, at least 88%, at least 90%, at least 95%, at least 98%, or 100% amino acid sequence identity; and   the enhanced trait is selected from the group consisting of smaller size, smaller petals, smaller sepals, increased tolerance to  Erysiphe , increased resistance to  Erysiphe , increased tolerance to  Botrytis , increased tolerance to  Sclerotinia , increased tolerance to  Pseudomonas , increased tolerance to salt, increased tolerance to sucrose, increased tolerance to glucose, increased tolerance to cold, increased tolerance in heat, increased tolerance to osmotic stress, increased tolerance to drought, increased tolerance to nutrient-limiting conditions, increased tolerance to nitrogen-limiting conditions, increased tolerance to phosphate-free medium, increased tolerance to potassium-free medium, increased tolerance to freezing, altered C:N sensing, reduced sensitivity to ABA, insensitive to ABA, decreased seed oil content, increased seed protein content, increased seed protein content, decreased seed protein, late flowering, early flowering, sterile, less pollen production, embryo lethal, delayed senescence, premature senescence, fewer trichomes at seedling stage, altered leaf shape, curled leaves, serrated leaves, shiny leaves, dark green leaves, dark green plant, light green coloration, reduced seed color, greater size, increased seedling size, large leaves, larger seeds, greater biomass, increased root hairs, homeotic transformation, terminal flowers, short stamen filaments, abnormal anther development, reduced fertility, glabrous, altered trichome distribution, increased trichome size, increased trichome density, reduced branching, more vascular bundles in stem, loss of apical dominance, long petioles, upturned leaves, smaller petals, smaller sepals, reduced or absent petals, sepals and stamens, reduced petal abscission, leaf cell expansion, slow growth, photomorphogenesis in dark conditions, lethal when overexpressed, increased leaf unsaturated fatty acids, increased leaf chlorophyll, increased leaf carotenoids, increased anthocyanins in leaf, increased anthocyanins in root, increased anthocyanins in seed, reduced lignin, increased lutein content, increased seed lutein, decreased seed lutein, decreased leaf lutein, increased xanthophyll, increased leaf xanthophyll, increased arabinose, increased leaf arabinose, increased mannose, increased δ- and γ-tocopherol, increased leaf insoluble sugars, decreased insoluble sugars, increase in xylose, increased leaf rhamnose, increased fucose, decreased rhamnose, increased leaf fatty acids, increase in glucosinolate M39480, increased glucosinolate M39481, increase in 18:1 fatty acids, increased seed 16:0 fatty acid, increased seed 16:1 fatty acid, increased seed 18:0 fatty acid, increased seed 18:1 fatty acid, increased seed 18:3 fatty acid, increased seed 20:0 fatty acid, reduction in 16:3 fatty acid, reduction in 18:2 fatty acids, reduction in 20:1 fatty acids, reduction in 22:1 fatty acids, increased wax in leaves, and, decreased seed fatty acid composition;   wherein when the polypeptide is expressed in the transgenic plant, the polypeptide confers to the trangenic plant the enhanced trait, as compared to the control plant.   
     
     
         6 . The method of  claim 5 , wherein the transgenic plant is dicotyledenous. 
     
     
         7 . The method of  claim 5 , wherein the transgenic plant is monocotyledenous. 
     
     
         8 . The method of  claim 5 , wherein expression of the polypeptide is regulated by a constitutive, inducible, or tissue-specific promoter. 
     
     
         9 . The method of  claim 5 , wherein the method further comprises the step of:
 (b) selfing or crossing the transformed plant with itself or another plant, respectively, to produce a transgenic seed.   
     
     
         10 . The method of  claim 5 , wherein the method further comprises the step of:
 (b) selecting the transgenic plant by its ectopic expression of the polypeptide or the presence of the enhanced trait, as compared to the control plant.