Preparation Comprising Insulin, Nicotinamide and an Amino Acid

Abstract

Insulin preparations comprising an insulin compound or a mixture of two or more insulin compounds, a nicotinic compound and an amino acid.

1 . An insulin preparation comprising:
 an insulin compound;   a nicotinic compound;   arginine; and   a buffer.   
     
     
         2 . The insulin preparation of  claim 1 , wherein the buffer is a phosphate buffer. 
     
     
         3 . The insulin preparation of  claim 2 , wherein the insulin compound comprises B28Asp human insulin. 
     
     
         4 . The insulin preparation of  claim 1 , wherein the insulin compound is selected from the group consisting of B28Asp human insulin, B28LysB29Pro human insulin, and B3LysB29Glu human insulin. 
     
     
         5 . The insulin preparation of  claim 4 , wherein the buffer is a phosphate buffer. 
     
     
         6 . An insulin preparation comprising:
 B28Asp human insulin;   nicotinamide;   zinc;   arginine; and   a phosphate buffer.   
     
     
         7 . The insulin preparation of  claim 6 , wherein the nicotinamide is present at a concentration ranging from about 1 mM to about 300 mM. 
     
     
         8 . The insulin preparation of  claim 7 , wherein less than about 4 zinc ions are present per six B28Asp human insulin molecules. 
     
     
         9 . The insulin preparation of  claim 8 , wherein the arginine is present in a concentration from about 10 mM to about 60 mM. 
     
     
         10 . The insulin preparation of  claim 9 , wherein the arginine is present in a concentration ranging from about 10 mM to about 40 mM. 
     
     
         11 . The insulin preparation of  claim 10 , wherein the preparation has a pH of about 7.4 or less. 
     
     
         12 . The insulin preparation of  claim 11 , wherein the preparation has a pH of about 7.1. 
     
     
         13 . The insulin preparation of  claim 6  wherein the nicotinamide is present at a concentration ranging from about 80 mM to about 260 mM. 
     
     
         14 . The insulin preparation of  claim 13 , wherein the arginine is present in a concentration ranging from about 10 mM to about 40 mM. 
     
     
         15 . The insulin preparation of  claim 14 , wherein the preparation has a pH of about 7.4 or less. 
     
     
         16 . The insulin preparation of  claim 15 , wherein less than about 4 zinc ions are present per six B28Asp human insulin molecules. 
     
     
         17 . The insulin preparation of  claim 14 , wherein the preparation has a pH of about 7.1. 
     
     
         18 . The insulin preparation of  claim 17 , wherein less than about 4 zinc ions are present per six B28Asp human insulin molecules.