Molecular markers linked to ppo inhibitor tolerance in soybeans

Abstract

This invention relates generally to the detection of genetic differences among soybeans. More particularly, the invention relates to soybean quantitative trait loci (QTL) for tolerance to protoporphyrinogen oxidase inhibitors, to soybean plants possessing these QTLs, which map to a novel chromosomal region, and to genetic markers that are indicative of phenotypes associated with protoporphyrinogen oxidase inhibitor tolerance. Methods and compositions for use of these markers in genotyping of soybean and selection are also disclosed.

1 - 11 . (canceled) 
     
     
         12 . A kit for selecting at least one soybean plant by marker assisted selection of a quantitative trait locus associated with tolerance to herbicides that inhibit protoporphyrinogen oxidase function comprising:
 a. chemically synthesized or chemically modified primers or probes for detecting at least one tolerance-associated marker locus, wherein the tolerance-associated locus is selected from the group consisting of S08102-1, S08103-1, S08104-1, S08106-1, S08107-1, S08109-1, S08110-1, S08111-1, S08115-2, S08117-1, S08119-1, S08116-1, S08112-1, S08108-1, S08101-4, S08101-1, S08101-2, S08101-3, S08105-1, S08118-1, S08010-1, S08010-2, S60167, P10649C, S00224-1, P5467, S04867-1 and S03859-1; and   b. instructions in using the primers or probes for detecting the marker loci and correlating the loci with predicted protoporphyrinogen oxidase inhibitor tolerance.   
     
     
         13 - 32 . (canceled) 
     
     
         33 . The kit of  claim 12 , wherein the tolerance-associated locus is detected using chemically synthesized or chemically modified primers or probes for a marker selected from the group consisting of S08102-1, S08103-1, S08104-1, S08106-1, S08107-1, S08109-1, S08110-1, S08111-1, S08115-2, S08117-1, S08119-1, S08116-1, S08112-1, S08108-1, S08101-4, S08101-1, S08101-2, S08101-3, S08105-1, S08118-1, S08010-1, S08010-2, S60167-TB, P10649C-3, S00224-1, P5467-1 and S04867-1 and S03859-1. 
     
     
         34 . The kit of  claim 12 , wherein the primers comprise a primer pair selected from the group consisting of:
 a. SEQ ID NO: 34 and SEQ ID NO: 35;   b. SEQ ID NO: 38 and SEQ ID NO: 39;   c. SEQ ID NO: 42 and SEQ ID NO: 43;   d. SEQ ID NO: 50 and SEQ ID NO: 51;   e. SEQ ID NO: 54 and SEQ ID NO: 55;   f. SEQ ID NO: 62 and SEQ ID NO: 63;   g. SEQ ID NO: 66 and SEQ ID NO: 67;   h. SEQ ID NO: 70 and SEQ ID NO: 71;   i. SEQ ID NO: 78 and SEQ ID NO: 79;   j. SEQ ID NO: 86 and SEQ ID NO: 87;   k. SEQ ID NO: 94 and SEQ ID NO: 95;   l. SEQ ID NO: 82 and SEQ ID NO: 83;   m. SEQ ID NO: 74 and SEQ ID NO: 75;   n. SEQ ID NO: 58 and SEQ ID NO: 59;   o. SEQ ID NO: 30 and SEQ ID NO: 31;   p. SEQ ID NO: 18 and SEQ ID NO: 19;   q. SEQ ID NO: 22 and SEQ ID NO: 23;   r. SEQ ID NO: 26 and SEQ ID NO: 27;   s. SEQ ID NO: 46 and SEQ ID NO: 47;   t. SEQ ID NO: 90 and SEQ ID NO: 91;   u. SEQ ID NO: 98 and SEQ ID NO: 99;   v. SEQ ID NO: 110 and SEQ ID NO: 111;   w. SED ID NO: 1 and SEQ ID NO: 2;   x. SEQ ID NO: 5 and SEQ ID NO: 6;   y. SEQ ID NO: 10 and SEQ ID NO: 11; and   z. SEQ ID NO: 14 and 15.   
     
     
         35 . The kit of  claim 12 , wherein the probes are selected from the group consisting of SEQ ID NOs: 7, 8, 9, 12, 13, 16, 17, 20, 21, 24, 25, 28, 29, 32, 33, 36, 37, 40, 41, 44, 45, 48, 49, 52, 53, 56, 57, 60, 61, 64, 65, 68, 69, 72, 73, 76, 77, 80, 81, 84, 85, 88, 89, 92, 93, 96, 97, 108, 109, 112, and 113.