Oocytes derived from ovarian culture initially containing no oocytes

Abstract

Ovarian germ-line-competent embryonic stem cells (GLC-ESC) are cultured, either in the presence or absence of a compound having estrogenic activity. The GLC-ESC are either collected prior to specific commitment or are permitted to remain in the culture medium for a time sufficient to develop into oocytes, and the oocytes may be fertilized by adding sperm to the culture medium. The fertilized oocytes may be permitted to develop into embryos, which may be transferred into the uterus of an adult human female or frozen for later use. The invention provides a method for obtaining by in vitro fertilization an embryo that is genetically related to a human female who is not producing oocytes.

1 . A method for obtaining a fertilized human oocyte comprising:
 culturing in a culture medium ovarian cells including surface epithelial cells and initially containing no oocytes;   permitting the cells to remain in the culture medium for a period of time sufficient for the cells to develop into oocytes;   adding sperm to the culture; and   permitting the sperm to associate with the ovarian cells, resulting in one or more fertilized oocytes.   
     
     
         2 . The method of  claim 1 , wherein the culture medium contains a source of estrogen. 
     
     
         3 . The method of  claim 2 , wherein the source of estrogen is a chemical compound having estrogenic activity. 
     
     
         4 . The method of  claim 3 , wherein the cells are permitted to remain in the culture medium for at least 4 to 6 days. 
     
     
         5 . The method of  claim 2 , wherein the source of estrogen is from ovarian cells in the culture that produce estrogen. 
     
     
         6 . The method of  claim 5 , wherein the cells are permitted to remain in the culture medium for at least 10 days. 
     
     
         7 . The method of  claim 1 , wherein the ovarian cells that are cultured were obtained from a postmenopausal or anovulatory ovary. 
     
     
         8 . The method of  claim 1 , further comprising permitting the fertilized oocytes to develop into one or more embryos. 
     
     
         9 . The method of  claim 8 , further comprising transferring one or more of the embryos into the uterus of an adult human female.