Method of cancer diagnosis using the analysis of isotopes

Abstract

Disclosed is a method of diagnosing cancer on the basis of the quantitative analysis of blood or tissue isotopes, for example, such as 36 S or 40 K. The method can accurately diagnose cancer in a patient even when it is too small for current conventional technology to diagnose.

1 . A method of diagnosing cancer in a patient, comprising
 (a) measuring the level of isotope  36 S in a blood sample or a tissue sample of the patient by using a double-focus type mass spectrophotometer;   (b) measuring the level of isotope  40 K in a blood sample or a tissue sample of the patient by using a double-focus type mass spectrophotometer; and   (c) diagnosing cancer in the patient if  36 S and  40 K are not detected.   
     
     
         2 . The method according to  claim 1 ,
 wherein the method further comprises measuring the level of deuterium  2 D or  18 O in a blood sample or a tissue sample of the patient and diagnosing cancer in the patient if the level of the  2 D or  18 O isotope is greater than the level of a normal standard for the  2 D or  18 O isotope.   
     
     
         3 . The method according to  claim 2 ,
 wherein the method comprises measuring the level of deuterium  2 D D in a blood sample or a tissue sample of the patient and diagnosing cancer in the patient if more than 1.2 times increased level of  2 D is detected in the blood sample or tissue sample of the patient compared to a normal standard for  2 D isotope, or the method comprises measuring the level of  18 O in a blood sample or a tissue sample of the patient and diagnosing cancer in the patient if more than 1.4 times increased level of  18 O is detected in the blood sample or tissue sample of the patient compared to a normal standard for  18 O isotope.   
     
     
         4 . The method according to  claim 1 ,
 wherein the method further comprises measuring the level of at least one from the group consisting of sulfur isotopes other than  36 S, magnesium isotopes, silicon isotopes, calcium isotopes and iron isotopes in a blood sample or a tissue sample of the patient and diagnosing cancer in the patient if an increased level of at least one from the group consisting of sulfur isotopes, magnesium isotopes, silicon isotopes, calcium isotopes and iron isotopes is detected in the blood sample or tissue sample of the patient compared to a normal standard for the isotope.   
     
     
         5 . The method according to  claim 4 ,
 wherein the method comprises measuring the level of sulfur isotope  33 S or  34 S in a blood sample or a tissue sample of the patient and diagnosing cancer in the patient if more than 1.3 times increased level of  33 S or  34 S is detected in the blood sample or tissue sample of the patient compared to a normal standard for  33 S or  34 S.   
     
     
         6 . The method according to  claim 4 ,
 wherein the method comprises measuring the level of  26 Mg in a blood sample or a tissue sample of the patient and diagnosing cancer in the patient if more than 2.5 times increased level of  26 Mg is detected in the blood sample or tissue sample of the patient compared to a normal standard for  26 Mg.   
     
     
         7 . The method according to  claim 4 ,
 wherein the method comprises measuring the level of  29 Si or  30 Si in a blood sample or a tissue sample of the patient and diagnosing cancer in the patient if more than 2.4 times increased level of  29 Si or  30 Si is detected in the blood sample or tissue sample of the patient compared to a normal standard for  29 Si or  20 Si.   
     
     
         8 . The method according to  claim 4 ,
 wherein the method comprises measuring the level of  57 Fe or  58 Fe in a blood sample or a tissue sample of the patient and diagnosing cancer in the patient if more than 1.6 times increased level of  57 Fe or  58 Fe is detected in the blood sample or tissue sample of the patient compared to a normal standard for  57 Fe or  99 Fe.   
     
     
         9 . The method according to  claim 4 ,
 wherein the method comprises measuring the level of at least one of the calcium isotopes  42 Ca,  43 Ca,  44 Ca,  46 Ca and  48 Ca in a blood sample or a tissue sample of the patient and diagnosing cancer in the patient if more than 2.8 times increased level of at least one of the calcium isotopes  42 Ca,  43 Ca,  44 Ca,  46 Ca and  48 Ca, is detected in the blood sample or tissue sample of the patient compared to a normal standard for at least one of the calcium isotopes  42 Ca,  43 Ca,  44 Ca,  46 Ca and  48 Ca.   
     
     
         10 . The method according to  claim 2 ,
 wherein the method comprises measuring the level of deuterium  2 D.   
     
     
         11 . The method according to  claim 2 ,
 wherein the method comprises measuring the level of  18 O.   
     
     
         12 . The method according to  claim 2 ,
 wherein the method further comprises measuring the level of at least one from the group consisting of sulfur isotopes other than  36 S, magnesium isotopes, silicon isotopes, calcium isotopes and iron isotopes in a blood sample or a tissue sample of the patient and diagnosing cancer in the patient if an increased level of at least one from the group consisting of sulfur isotopes, magnesium isotopes, silicon isotopes, calcium isotopes and iron isotopes is detected in the blood sample or tissue sample of the patient compared to a normal standard for the isotope.   
     
     
         13 . The method according to  claim 3 ,
 wherein the method further comprises measuring the level of at least one from the group consisting of sulfur isotopes other than  36 S, magnesium isotopes, silicon isotopes, calcium isotopes and iron isotopes in a blood sample or a tissue sample of the patient and diagnosing cancer in the patient if an increased level of at least one from the group consisting of sulfur isotopes, magnesium isotopes, silicon isotopes, calcium isotopes and iron isotopes is detected in the blood sample or tissue sample of the patient compared to a normal standard for the isotope.