Perfusion Culture Method and Perfusion Culture Device for Organ or Tissue

Abstract

Disclosed are a perfusion culture method and a perfusion culture device whereby a perfusate can be delivered to all parts of an organ or tissue so that the organ or tissue can be preserved while sufficiently maintaining the function thereof. Specifically disclosed is a method for perfusion culture of an organ or tissue removed from a mammal, said organ or tissue having been removed together with a second organ or tissue connected to the aforesaid organ or tissue in vivo, which comprises a step for fixing said second organ or tissue so as to hang the aforesaid organ or tissue and a step for perfusing blood vessels in the aforesaid organ or tissue with a perfusate.

1 . A perfusion culture device for an organ or tissue, comprising:
 a suspension means for hanging the organ or tissue,   a perfusate inflow cannula for allowing a perfusate to flow into the organ or tissue, and   a perfusate outflow cannula for allowing the perfusate to flow out from the organ or tissue.   
     
     
         2 . The perfusion culture device according to  claim 1 , further comprising a vessel that can allow immersion of at least a part of the organ or tissue in the liquid in a state that the organ or tissue is hanged. 
     
     
         3 . The perfusion culture device according to  claims 1 , wherein the organ or tissue is a liver, and further comprising a biliary cannula. 
     
     
         4 . The perfusion culture device according to  claim 1 ;
 wherein the organ or tissue is a liver, and   wherein the said suspension means has a configuration where the liver extirpated together with a rib and a diaphragm from a mammal is fixable at the rib.   
     
     
         5 . The perfusion culture device according to  claim 1 , wherein the organ or tissue is a kidney, and further comprising a ureteral cannula. 
     
     
         6 . The perfusion culture device according to  claim 1 , wherein:
 the organ or tissue is a kidney, and   the suspension means has a configuration where the kidney extirpated together with the surrounding adipose tissue from a mammal is fixable at the adipose tissue.   
     
     
         7 . The perfusion culture device according to  claim 2 , wherein the organ or tissue is a liver, and further comprising a biliary cannula. 
     
     
         8 . The perfusion culture device according to  claim 2 ,
 wherein the organ or tissue is a liver, and   wherein said suspension means has a configuration where the liver extirpated together with a rib and a diaphragm from a mammal is fixable at the rib.   
     
     
         9 . The perfusion culture device according to  claim 3 ,
 wherein the organ or tissue is a liver, and   wherein said suspension means has a configuration where the liver extirpated together with a rib and a diaphragm from a mammal is fixable at the rib.   
     
     
         10 . The perfusion culture device according to  claim 2 , wherein the organ or tissue is a kidney, and further comprising a ureteral cannula. 
     
     
         11 . The perfusion culture device according to  claim 2 , wherein
 the organ or tissue is a kidney, and   the suspension means has a configuration where the kidney extirpated together with the surrounding adipose tissue from a mammal is fixable at the adipose tissue.   
     
     
         12 . The perfusion culture device according to  claim 5 , wherein the organ or tissue is a kidney, and
 the suspension means has a configuration where the kidney extirpated together with the surrounding adipose tissue from a mammal is fixable at the adipose tissue.