Multi-array, multi-specific electrochemiluminescence testing

Abstract

Materials and Methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.

1 - 53 . (canceled) 
     
     
         54 . An insrumen system for conducting elecrochemiluminescence assays for the deecion or quaniaion of an analye comprising:
 (a) a cartridge including one or more electrodes each comprising a composite containing a polymer matrix and a muftipliciy of carbon particles dispersed therein, each of said one or more electrodes having one or a plurality of binding domains, each of said domains containing a reagent capable of binding a component of a binding elecrochemiluminescence assay; and   (b) means for selectively deecing or quaniaing light from each of said binding domains.   
     
     
         55 . An insrumen system as recited in  claim 54 , wherein said carbon particles are fibrils. 
     
     
         56 . An insrumen system as recited in  claim 54 , including means for mixing conens of said cartridge. 
     
     
         57 . An insrumen system as recited in  claim 54 , wherein said cartridge includes a dry assay reagenL 
     
     
         58 . An insrumen as recited in  claim 54 , wherein said means for detecting or quaniaing light is a CCD. 
     
     
         59 . An insrumen as recited in  claim 54 , wherein said means for detecting or quaniaing light is a phoodiode. 
     
     
         60 . An insrumen as recited in  claim 54 , including temperaure control means for controlling the temperature of said cartridge. 
     
     
         61 . An insrumen system for conducting elecrochemiluminescence assays for the deecion or quaniaion of an analyte comprising:
 (a) a cartridge including one or more electrodes each comprising a composite containing a matrix and a muftipliciy of carbon fibrils dispersed therein, each of said one or more electrodes having one or a plurality of binding domains, each of said domains containing a reagent capable of binding a component of a binding elecrochemiluminescence assay;   (b) means for selectively deecing or quaniaing light from each of said binding domains; and   (c) means for mixing conens of said cartridge.   
     
     
         62 . An insrumen system as recited in  claim 61 , wherein said mixing system is a sonicaion device. 
     
     
         63 . An instrument system for conducting elecrochemiluminescence assays for the deecion or quaniaion of an analye comprising:
 (a) a cartridge including one or more electrodes each comprising a composite containing a matrix and a muftipliciy of carbon particles dispersed therein, each of said one or more electrodes having one or a plurality of binding domains, each of said domains containing a reagent capable of binding a component of a binding elecrochemiluminescence assay;   (b) means for applying a poenial a one or more of said electrodes;   (c) means for selectively deecing or quaniaing light from said binding domains;   (d) electronic means o coordinate he application of a poenial a one or more of said electrodes with he operation of said means for deecing or quaniaing light from said binding domains; and   (e) electronic means for storage or processing of information received form said light deecing or quaniaion means.   
     
     
         64 . An insrumen system as recited in  claim 63 , including means for mixing conens of said cartridge. 
     
     
         65 . An insrumen system as recited in  claim 63 , wherein said cartridge includes a dry assay reagent. 
     
     
         66 . An instrument system as recited in  claim 63 , wherein said carbon particles are fibrils. 
     
     
         67 . An instrument system for conducting elecrochemiluminescence assays for the deecion or quaniaion of an analye, comprising:
 (a) a cartridge including one or more electrodes each of said one or more electrodes having one or a plurality of binding domains, each of said domains containing a reagent capable of binding a component of a binding elecrochemiluminescence assay;   (b) means for applying a potential a one or more of said electrodes;   (c) means for selectively deecing or quaniaing light from said binding domains;   (d) electronic means o coordinate he application of a poenial a one or more of said electrodes with he operation of said means for detecting or quaniaing light from said binding domains; and   (e) electronic means for storage or processing of information received form said light deecing or quantitation means.   
     
     
         68 . An instrument system as recited in  claim 67 , including means for mixing the conens of said cartridge. 
     
     
         69 . An insrumen as recited in  claim 67 , including temperature control means for controlling he emperaure of said cartridge. 
     
     
         70 . An instrument system as recited in  claim 67 , wherein said cartridge includes a dry assay reagent. 
     
     
         71 - 108 . (canceled)