Method of fabricating test strip of biological fluid

Abstract

The invention provides a method of fabricating test strips for measuring biological blood. The method comprises steps: preparing an insulating membrane material; printing a metallic circuit layer on the insulating membrane material by a metallic ionic ink; performing a surface treatment for the metallic circuit layer expose metallic ions of the metallic ionic ink; chemical plating for the exposed metallic ions of the metallic circuit layer to form an electrode section on the metallic circuit layer; providing a sensing reagent on the electrode section to form a sensing reagent layer; sequentially adhering an intermediate layer and a cover to the insulating membrane material to cover the metallic circuit layer and expose part of the metallic circuit layer; and cutting the insulating membrane material to form a plurality of insulating sheets. The test strip is provided to enhance the stability of resistance and the test accuracy.

What is claimed is: 
     
         1 . A method of fabricating test strips for measuring biological blood comprising steps of:
 (a) preparing an insulating membrane material;   (b) printing a metallic circuit layer on the insulating membrane material by a metallic ionic ink;   (c) performing a surface treatment for the metallic circuit layer to expose metallic ions of the metallic ionic ink by treating the insulating membrane material printed with the metallic ionic ink;   (d) performing a chemical plating for the exposed metallic ions of the metallic circuit layer to form an electrode section on the metallic circuit layer;   (e) providing a sensing reagent on the electrode section to form a sensing reagent layer;   (f) adhering an intermediate layer to the insulating membrane material to cover the metallic circuit layer and expose part of the metallic circuit layer, the intermediate layer having an opening to expose the sensing reagent layer and the electrode section; and   (g) adhering a cover to cover the intermediate layer and the insulating membrane material and expose part of the metallic circuit layer, the cover having two holes corresponding to the opening.   
     
     
         2 . The method of fabricating a test strip for measuring biological fluid according to  claim 1 , wherein the insulating membrane material of step (a) is paper, timber, Acrylonitrile-Butadiene—Styrene (ABS), polycarbonate (PC), a mixture of ABS and PC, polyethylene terephthalate (PET), polyimide (PI), thermoplastic elastomer or polylactic acid plastic. 
     
     
         3 . The method of fabricating a test strip for measuring biological fluid according to  claim 2 , wherein the surface treatment of step (c) of the metallic circuit layer is performed by baking or immersing into an acidic solution to melt or etch resins disposed a surface of the metallic ionic ink and expose the metallic ions. 
     
     
         4 . The method of fabricating a test strip for measuring biological fluid according to  claim 3 , wherein the chemical plating of the step (d) is performed by metallic ion replacement or electroless plating with immersing the exposed metallic ions into an active solution including metallic ions which are different to the exposed metallic ions to replace the metallic ions of the metallic ionic ink with the metallic ions of the active solution. 
     
     
         5 . The method of fabricating a test strip for measuring biological fluid according to  claim 4 , wherein the metallic circuit layer includes an electrical connector, a metallic circuit connecting to the electrical connector and an electrode section connecting to an end of the metallic circuit. 
     
     
         6 . The method of fabricating a test strip for measuring biological fluid according to  claim 5 , wherein forming a sensing reagent layer of the step (e) is performed by spray marking the sensing reagent on the electrode section to form a sensing reagent layer. 
     
     
         7 . The method of fabricating a test strip for measuring biological fluid according to  claim 6 , wherein the intermediate layer of the step (f) is adhered to the insulating membrane material to cover the metallic circuit and the electrode section of the metallic circuit layer and expose the electrical connector. 
     
     
         8 . The method of fabricating a test strip for measuring biological fluid according to  claim 7 , wherein the intermediate layer is shorter than the insulating membrane material and the intermediate layer is of insulating material. 
     
     
         9 . The method of fabricating a test strip for measuring biological fluid according to  claim 8 , wherein a cover of the step (g) is covered on the intermediate layer to expose the electrical connector of the metallic circuit layer, the cover is shorter than the insulating membrane material and the cover is of insulating material. 
     
     
         10 . The method of fabricating a test strip for measuring biological fluid according to  claim 9 , after the step (g) further comprising cutting the insulating membrane material to form a plurality of insulating sheets, each of the insulating sheets having the metallic circuit layer, the sensing reagent layer, the intermediate layer and the cover. 
     
     
         11 . The method of fabricating a test strip for measuring biological fluid according to  claim 10 , wherein the resistance of the metallic circuit layer has a range from 0.01 Ω to 2 k Ω. 
     
     
         12 . The method of fabricating a test strip for measuring biological fluid according to  claim 11 , wherein the thickness of the metallic circuit layer has a range from 0.02 um to 30 um.