Preparation of Regenerative Tissue Scaffolds

Abstract

Devices and methods for treating or repairing a tissue or organ defect or injury are provided. The devices can include tissue scaffolds produced from acellular tissue matrices and polymers, which have a stable three-dimensional shape and elicit a limited immunologic or inflammatory response.

1 . A method for making a tissue scaffold, comprising:
 dissolving a polymer in a solvent to make a solution;   mixing the solution with a particulate acellular tissue matrix (ATM) to create a mixture;   placing the mixture in a mold; and   drying the mixture to form a tissue scaffold with a stable three-dimensional shape, wherein the tissue scaffold has a reduced immunological or inflammatory response when implanted in a human than the polymer alone.   
     
     
         2 . The method of  claim 1 , wherein the polymer comprises a polycaprolactone. 
     
     
         3 . The method of  claim 2 , wherein the solvent comprises dioxane. 
     
     
         4 . The method of  claim 2 , wherein the solvent comprises N-methyl-2-pyrrolidone. 
     
     
         5 . The method of  claim 2 , wherein the polycaprolactone in solvent is present in an amount ranging from about 5-30% (w/v). 
     
     
         6 . The method of  claim 2 , wherein the polycaprolactone in solvent is present in an amount ranging from about 10-30% (w/v). 
     
     
         7 . The method of  claim 1 , wherein the polymer is a poly-4-hydroxybutyrate. 
     
     
         8 . The method of  claim 7 , wherein the solvent comprises dioxane. 
     
     
         9 . The method of  claim 7 , wherein the solvent comprises N-methyl-2-pyrrolidone. 
     
     
         10 . The method of  claim 7 , wherein the poly-4-hydroxybutyrate in solvent is present in an amount ranging from about 5-40% (w/v). 
     
     
         11 . The method of  claim 7 , wherein the poly-4-hydroxybutyrate in solvent is present in an amount ranging from about 10-30% (w/v). 
     
     
         12 . The method of  claim 1 , wherein the polymer comprises a benzyl ester derivative of hyaluronic acid. 
     
     
         13 . The method of  claim 12 , wherein the solvent comprises DMSO. 
     
     
         14 . The method of  claim 12 , wherein the benzyl ester derivative of hyaluronic acid in solvent is present in an amount ranging from about 5-50% (w/v). 
     
     
         15 . The method of  claim 12 , wherein the benzyl ester derivative of hyaluronic acid in solvent is present in an amount ranging from about 10-40% (w/v). 
     
     
         16 . The method of  claim 1 , wherein the polymer comprises chitosan. 
     
     
         17 . The method of  claim 16 , wherein the solvent comprises acetic acid. 
     
     
         18 . The method of  claim 17 , wherein the acetic acid is 0.1-0.5 M. 
     
     
         19 . The method of  claim 16 , wherein the chitosan is present in an amount ranging from about 1-5% (w/v). 
     
     
         20 . The method of  claim 1 , wherein the particulate ATM comprises uniform size particles. 
     
     
         21 . The method of  claim 1 , wherein the particulate ATM comprises a dermal ATM. 
     
     
         22 . The method of  claim 21 , wherein the dermal ATM is a human tissue matrix. 
     
     
         23 . The method of  claim 21 , wherein the dermal ATM is a porcine tissue matrix. 
     
     
         24 . The method of  claim 1 , wherein the particulate ATM is a cartilage tissue matrix. 
     
     
         25 . The method of  claim 24 , wherein the cartilage tissue matrix comprises a human cartilage matrix. 
     
     
         26 . The method of  claim 24 , wherein the cartilage tissue matrix comprises a porcine cartilage matrix. 
     
     
         27 . The method of  claim 1 , wherein the particulate ATM comprises a bone tissue matrix. 
     
     
         28 . The method of  claim 27 , wherein the bone tissue matrix comprises a human bone. 
     
     
         29 . The method of  claim 27 , wherein the bone tissue matrix comprises a porcine bone. 
     
     
         30 . The method of  claim 1 , wherein placing the mixture in a mold comprises injection molding. 
     
     
         31 . The method of  claim 1 , wherein the particulate ATM comprises ATM from two or more different types of tissues. 
     
     
         32 . The method of  claim 31 , wherein the two or more different types of tissues comprise dermis and cartilage. 
     
     
         33 . The method of  claim 31 , wherein the two or more different types of tissues comprise cartilage and bone. 
     
     
         34 . The method of  claim 31 , wherein the two or more different types of tissues comprise human tissue matrices. 
     
     
         35 . The method of  claim 31 , wherein the two or more different types of tissues comprise porcine tissue matrices. 
     
     
         36 . The method of  claim 31 , wherein the two or more different types of tissues comprise human tissue matrix and porcine tissue matrix. 
     
     
         37 .- 55 . (canceled) 
     
     
         56 . A regenerative tissue scaffold made according to the method of  claim 1 .