Process for concentration of a polypeptide

Abstract

The present invention comprises a method of concentrating a composition comprising a polypeptide of interest and the use of such a concentrated composition for the treatment of diseases in mammals, in particular by subcutaneous injection.

1 - 28 . (canceled) 
     
     
         29 . A composition comprising 50-300 mg/ml Aryl sulfatase A for the manufacture of a medicament for subcutaneous injection into a mammal. 
     
     
         30 . A method of treating a mammal for metachromatic leukodystrophy comprising a subcutaneous injection of a composition according to  claim 29 . 
     
     
         31 . A composition comprising 50-300 mg/ml porphobilinogen deaminase for the manufacture of a medicament for subcutaneous injection into a mammal. 
     
     
         32 . A method of treating a mammal for porphobilinogen deaminase acute intermittent porphyria comprising a subcutaneous injection of a composition according to  claim 31 . 
     
     
         33 . A composition comprising 50-300 mg/ml alpha-mannosidase for the manufacture of a medicament for subcutaneous injection into a mammal. 
     
     
         34 . A method of treating a mammal for the lysosomal storage disorder alpha-mannosidosis comprising a subcutaneous injection of a composition according to  claim 33 . 
     
     
         35 . A composition comprising 50-300 mg/ml galactosylcerebrosidase for the manufacture of a medicament for subcutaneous injection into a mammal. 
     
     
         36 . A method of treating a mammal for Krabbe disease comprising a subcutaneous injection of a composition according to  claim 35 . 
     
     
         37 . A method of concentrating a composition comprising Aryl sulfatase A, functionally equivalent parts and analogues hereof, said method comprising:
 (i) centrifugation and/or filtration of a composition comprising Aryl sulfatase A and/or a functionally equivalent part or analogue hereof, to obtain a resulting solution; and   (ii) concentrating said resulting solution.   
     
     
         38 . A method of concentrating a composition comprising porphobilinogen deaminase, functionally equivalent parts and analogues hereof, said method comprising:
 (i) centrifugation and/or filtration of a composition comprising prophobilinogen deaminase and/or a functionally equivalent part or analogue hereof, to obtain a resulting solution; and   (ii) concentrating said resulting solution.   
     
     
         39 . A method of concentrating a composition comprising alfa mannosidase, functionally equivalent parts and analogues hereof, said method comprising:
 (i) centrifugation and/or filtration of a composition comprising alfa mannosidase and/or a functionally equivalent part or analogue hereof, to obtain a resulting solution; and   (ii) concentrating said resulting solution.   
     
     
         40 . A method of concentrating a composition comprising galactosylcerebrosidase, functionally equivalent parts and analogues hereof, said method comprising:
 (i) centrifugation and/or filtration of a composition comprising galactosylcerebrosidase and/or a functionally equivalent part or analogue hereof, to obtain a resulting solution; and   (ii) concentrating said resulting solution.