Novel lipolytic enzyme lip2

Abstract

The present invention provides a novel nucleic acid sequence, designated LIP2, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same.

1 . A substantially pure polypeptide, wherein said polypeptide comprises an amino acid sequence having at least 75% amino acid sequence identity with an amino acid sequence set forth in SEQ ID NO: 3, wherein said polypeptide has lipolytic activity. 
     
     
         2 . The polypeptide of  claim 1 , wherein said polypeptide comprises an amino acid sequence having at least 85% sequence identity with an amino acid sequence set forth in SEQ ID NO: 3. 
     
     
         3 . The polypeptide of  claim 1 , wherein said polypeptide comprises an amino acid sequence having at least 95% sequence identity with an amino acid sequence set forth in SEQ ID NO: 3. 
     
     
         4 . The polypeptide of  claim 1 , wherein said polypeptide comprises an amino acid sequence having at least 75% sequence identity with an amino acid sequence set forth in SEQ ID NO:3 and is encoded by a polynucleotide having a nucleic acid sequence having at least 75% nucleic acid sequence identity with a nucleic acid sequence set forth in SEQ ID NO: 1. 
     
     
         5 . The polynucleotide of  claim 4 , wherein said polynucleotide comprises a nucleic acid sequence having at least 85% nucleic acid sequence identity with a nucleic acid sequence set forth in SEQ ID NO: 1. 
     
     
         6 . The polynucleotide of  claim 4 , wherein said polynucleotide comprises a nucleic acid sequence having at least 95% nucleic acid sequence identity with a nucleic amino acid sequence set forth in SEQ ID NO: 1. 
     
     
         7 . The polynucleotide of  claim 4 , wherein said polynucleotide encoding a polypeptide is shown in  FIG. 3 . 
     
     
         8 . A nucleic acid construct comprising the nucleic acid sequence of  claim 4 , said nucleic acid sequence, which encodes for a polypeptide, being operably linked to one or more control sequences that direct the production of said polypeptide in a suitable host. 
     
     
         9 . A recombinant expression vector comprising the nucleic acid construct of  claim 8 . 
     
     
         10 . A recombinant host cell comprising the nucleic acid construct of  claim 8 . 
     
     
         11 . The host cell of  claim 10 , wherein said host cell is  E. coli.    
     
     
         12 . A detergent composition comprising the polypeptide of  claim 1 . 
     
     
         13 . A method for producing a lipolytic enzyme comprising
 a) transforming a host cell with a nucleic acid encoding a lipolytic enzyme; said nucleic acid having at least 75% nucleic acid sequence identity with a nucleic acid sequence as set forth in SEQ ID NO: 1;   b) culturing the host cell under conditions to produce the lipolytic enzyme and recovering the lipolytic enzyme.