Platelet lysate gel

Abstract

The invention concerns a pharmaceutical composition comprising a platelet lysate and its use to treat a wound, an anal fissure, vaginal atrophy or a wrinkle.

1 - 11 . (canceled) 
     
     
         12 . A method of producing a platelet lysate comprising subjecting a population of platelets to at least one freeze-thaw cycle, wherein the freeze portion of each cycle is carried out at a temperature lower than or equal to −78° C. 
     
     
         13 . A method according to  claim 12 , wherein the method comprises subjecting the population of platelets to three or four freeze-thaw cycles. 
     
     
         14 . A method according to  claim 12 , wherein liquid nitrogen is used as a cryogenic means whereby immersion in the freeze portion of each cycle results in 95% or more lysis of platelets resulting in greater growth factor release and improved function in cell growth, repair and regeneration measurable by but not limited to fibroblast and PGDF assay. 
     
     
         15 . A method according to  claim 12 , where dry ice (solid carbon dioxide) and an organic solvent are used as a cryogenic means in the freeze portion of each cycle. 
     
     
         16 . A method of producing platelet lysate comprising subjecting a population of platelets to mechanical homogenisation (‘waring blender’), liquid homogenization, sonication, freeze-thaw using alcohol and dry ice or other means, mechanical grinding in liquid nitrogen at >75%, but typically >90%, lysis to release growth factors from platelets and alpha granules into solution whereas rotating blades grind and disperse cells and tissues; cell or tissue suspensions are sheared by forcing them through a narrow space; high frequency sound waves shear cells; repeated cycles of freezing and thawing disrupt cells through ice crystal formation; grinding tissue, frozen in liquid nitrogen wherein lysis releases growth factors from platelets and alpha granules into solution. 
     
     
         17 . A method according to  claim 12  or  16 , wherein the method further comprises lyophilising the platelet lysate. 
     
     
         18 . A platelet lysate produced using a method according to  claim 12  or  16 . 
     
     
         19 . A platelet lysate according to  claim 18  for use in treating a wound, an anal fissure, vaginal atrophy or a wrinkle in a patient in need thereof. 
     
     
         20 . A method of treating a wound, an anal fissure, vaginal atrophy or a wrinkle in a patient in need thereof, comprising administering to the patient a therapeutically effective amount of a platelet lysate according to  claim 18 . 
     
     
         21 - 22 . (canceled)