Brassica Ogura Restorer Lines with Shortened Raphanus Fragment (SRF)

Abstract

New Brassica Ogura fertility restorer lines with a shortened Raphanus fragment are provided. The new lines lack the OPC2 marker and are capable of fully restoring fertility in Ogura cytoplasmic male sterile (cms) plants. The improved lines were developed using a new breeding method. The new breeding method can be used to shorten an exotic insertion comprising a gene of interest in any plant.

1 . A  Brassica  plant comprising a fertility gene for  Ogura  cytoplasmic male sterility, wherein the fertility gene is on a  Raphanus  fragment introgressed from  Raphanus sativa , and the  Raphanus  fragment lacks a marker selected from the group consisting of RMA01, RMA02, RMA03, RMA04, RMA05, RMA06, RMA07, RMA08, RMA09, RMA10, RMC24, OPC2, RMC25, RMC26, RMC27, RMC28, RMC29, RMC30, RMC31, RMC32 and RMC33. 
     
     
         2 . The  Brassica  plant of  claim 1  wherein the  Raphanus  fragment lacks the OPC2 marker. 
     
     
         3 . (canceled) 
     
     
         4 . The  Brassica  plant of  claim 1  wherein the  Raphanus  fragment comprises a molecular marker selected from the group consisting of RMB01, E35M62, RMB02, RMB03, RMB04, RMB05, RMB06, RMB07, RMB08, RMB09, RMB10, OPF10, RMB11, RMB12, RMC01, RMCO2, RMCO3, E38M60, RMC04, RMCO5, RMC06, RMC07, RMC08, RMC09, RMC10, RMC11, RMC12, RMC13, RMC14, RMC15, RMC16, RMC17, RMC18, RMC19, RMC20, RMC21, RMC22 AND RMC23. 
     
     
         5 . (canceled) 
     
     
         6 . (canceled) 
     
     
         7 . (canceled) 
     
     
         8 . The  Brassica  plant of  claim 4  designated R1815, representative seed of which have been deposited under NCIMB Accession Number 41511, or a descendent or a plant produced by crossing R1815 with a second plant. 
     
     
         9 . A progeny or descendent plant of the  Brassica  plant of  claim 8 , wherein the progeny or descendent plant comprises a  Raphanus  fragment which lacks a marker selected from the group consisting of RMA01, RMA02, RMA03, RMA04, RMA05, RMA06, RMA07, RMA08, RMA09, RMA10, RMC24, OPC2, RMC25, RMC26, RMC27, RMC28, RMC29, RMC30, RMC31, RMC32 and RMC33. 
     
     
         10 - 14 . (canceled) 
     
     
         15 . A plant cell from the  Brassica  plant of  claim 1 . 
     
     
         16 . A part of the  Brassica  plant of  claim 1 . 
     
     
         17 - 42 . (canceled) 
     
     
         43 . A  Brassica  plant comprising the recombination event of R1815. 
     
     
         44 . (canceled)