Apparatus for oxygenation and perfusion of tissue for organ preservation

Abstract

An apparatus according to an embodiment is configured to oxygenate and perfuse a bodily tissue for extracorporeal preservation of the bodily tissue. The apparatus includes a pneumatic system, a pumping chamber, and an organ chamber. The pneumatic system is configured for the controlled delivery of fluid to and from the pumping chamber based on a predetermined control scheme. The predetermined control scheme can be, for example, a time-based control scheme or a pressure-based control scheme. The pumping chamber is configured to diffuse a gas into a perfusate and to generate a pulse wave for moving the perfusate through a bodily tissue. The organ chamber is configured to receive the bodily tissue and the perfusate. The organ chamber is configured to substantially automatically purge excess fluid from the organ chamber to the pumping chamber. The pumping chamber is configured to substantially automatically purge excess fluid from the pumping chamber to an area external to the apparatus.

What is claimed is: 
     
         1 - 25 . (canceled) 
     
     
         26 . A pneumatic control system for a portable organ perfusion device including a pumping chamber, the control system comprising:
 a supply line adapted to be connected to a source of an oxygen-containing gas;   a vent line providing a fluidic pathway to an exterior of a portable organ perfusion device;   a control line providing a fluidic path to a pumping chamber of the portable organ perfusion device;   a valve fluidicly coupled to the supply line, the vent line, and the control line; and   a control circuit operably connected to the valve, and configured to be connected to a power source,   wherein the control circuit instructs the valve to move between a first configuration in which a fluidic path is provided between the source of an oxygen-containing gas and the pumping chamber and a second configuration in which a fluidic path is provided between the pumping chamber and the exterior of the portable organ perfusion device.   
     
     
         27 . The pneumatic control system of  claim 26 , wherein the valve is a solenoid valve configured to move between the second and first configuration when the solenoid valve receives an electronic signal from the control circuit. 
     
     
         28 . The pneumatic control system of  claim 26 , wherein the control system is configured to energize the solenoid valve, causing it to move into the first position, and wherein the solenoid valve returns to the second position when the valve is not energized. 
     
     
         29 . The pneumatic control system of  claim 26 , further comprising a control orifice fluidicly coupled to the valve and located between the valve and the pumping chamber and configured to control the rate of flow between the valve and the pumping chamber. 
     
     
         30 . The pneumatic control system of  claim 29 , wherein the control orifice is a needle valve having an orifice between 0.1 mm and 0.6 mm in size. 
     
     
         31 . The pneumatic control system of  claim 26 , wherein the controller is configured to cause the valve to move between the first and second configurations after about 170 milliseconds. 
     
     
         32 . The pneumatic control system of  claim 30 , wherein the controller is configured to cause the valve to move between the second and first configurations after about 700 milliseconds. 
     
     
         33 . The pneumatic control system of  claim 26 , wherein the system is configured to receive an oxygenated gas from the source at a pressure of about 2±0.2 pounds per square inch above atmospheric pressure (PSIG). 
     
     
         34 . The pneumatic control system of  claim 26 , wherein the portable organ perfusion device comprises a pressure sensor, and the pressure sensor is configured to send a pressure reading to the control circuit. 
     
     
         35 . The pneumatic control system of  claim 34 , wherein the control circuit is configured to adjust a time that the valve is in the first configuration based upon the pressure reading. 
     
     
         36 . The pneumatic control system of  claim 34 , wherein the control circuit is configured to cause the valve to move between the first configuration and the second configuration when a predetermined pressure is measured within the portable organ perfusion device. 
     
     
         37 . The pneumatic control system of  claim 36 , wherein the control circuit causes the valve to move from the first configuration to the second configuration when the measured pressure is greater than about 20 mmHg above atmospheric pressure. 
     
     
         38 . The pneumatic control system of  claim 37 , wherein the control circuit causes the valve to move from the first configuration to the second configuration when the measured pressure is greater than about 40 mmHg above atmospheric pressure. 
     
     
         39 . The pneumatic control system of  claim 36 , wherein the control circuit causes the valve to move from the second configuration to the first configuration when the measured pressure is less than about 10 mmHg above atmospheric pressure. 
     
     
         40 . The pneumatic control system of  claim 39 , wherein the control circuit causes the valve to move from the second configuration to the first configuration when the measured pressure is about atmospheric pressure. 
     
     
         41 . The pneumatic control system of  claim 26 , wherein the controller is configured to calculate an organ flow rate and adjust a duration of the first or the second configuration based upon the measured organ flow rate.