Process for purification and isolation of estrogens

Abstract

The present application relates to a method for obtaining a mixture of estrogens from the pregnant mare's urine.

1 . A method for obtaining a mixture of estrogens, comprising:
 (a) adsorbing a mixture of estrogens from pregnant mare's urine on a resin;   (b) washing the resin loaded with the mixture of estrogens with water immiscible organic solvent;   (c) isolating the mixture of estrogens from the resin loaded with the mixture of estrogens.   
     
     
         2 . The method according to  claim 1 , wherein the resin is selected from an ion exchange resin, a non-ionic resin, synthetic adsorbents or combinations thereof. 
     
     
         3 . The method according to  claim 2 , wherein the resin is a cation exchange resin. 
     
     
         4 . The method according to  claim 2 , wherein the resin is an anion exchange resin. 
     
     
         5 . The method according to  claim 2 , wherein the resin is a non-ionic resin. 
     
     
         6 . The method according to  claim 2 , wherein the resin is a chelate resin. 
     
     
         7 . The method according to  claim 2 , where in the resin is lipophilic, hydrophilic and/or hydrophobic in nature. 
     
     
         8 . The method according to  claim 2 , wherein the resin is selected from aromatic type adsorbents, modified aromatic type adsorbents, methacrylic type adsorbents or combinations thereof. 
     
     
         9 . The method according to  claim 8 , wherein the modified aromatic type adsorbent resin is modified styrene-divinylbenzene polymer based resin. 
     
     
         10 . The method according to  claim 9 , wherein the modified styrene-divinylbenzene polymer based resin is brominated styrene-divinylbenzene polymer based resin. 
     
     
         11 . The method according to  claim 8 , wherein the resin is selected from HP20, HP21, SP825L, SP850, SP70, SP700, SP207, HP2MG or combinations thereof. 
     
     
         12 . The method according to  claim 11 , wherein the resin is selected from HP20, SP207 or combinations thereof. 
     
     
         13 . The method according to  claim 12 , wherein the resin is HP20. 
     
     
         14 . The method according to  claim 12 , wherein the resin is SP207. 
     
     
         15 . The method according to  claim 1 , wherein the water immiscible organic solvent is selected from halogenated hydrocarbon solvents, aromatic hydrocarbon solvents, aliphatic or alicyclic hydrocarbon solvents, ether solvents or mixtures thereof. 
     
     
         16 . The method according to  claim 15 , wherein the organic solvent is selected from halogenated hydrocarbon solvents, aromatic hydrocarbon solvents, ether solvents or mixture thereof. 
     
     
         17 . The method according to  claim 16 , wherein the organic solvent is selected from dichloromethane, chloroform, 1,2-dicholroethane, 1,1,2-trichloroethane, toluene, chlorobenzene, xylene, diethyl ether, diisopropyl ether, tert-butyl methyl ether or mixtures thereof. 
     
     
         18 . The method according to  claim 1 , wherein the resin loaded with the estrogens is washed with water immiscible organic solvent at a temperature of less than about 50° C. 
     
     
         19 . The method according to  claims 1 , wherein the resin loaded with the estrogens is washed with water immiscible organic solvent at a temperature of less than about 40° C. 
     
     
         20 . The method according to  claims 1 , wherein the resin loaded with the estrogens is washed with water immiscible organic solvent at a temperature of less than about 30° C. 
     
     
         21 . The method according to  claim 1 , wherein the isolating step comprises eluting the mixture of estrogens form the resin loaded with the mixture of estrogens with a solvent and removal of the solvent from the eluate. 
     
     
         22 . The method according  claim 21 , wherein the solvent is selected from alcohol, ketone, nitrile, ester, water or mixtures thereof. 
     
     
         23 . The method according to  claim 22 , wherein the solvent is selected from methanol, ethanol, 1-propanol, 2-propanol, acetone, propanone, 1-butanone, 2-butanone, acetonitrile, water or mixtures thereof. 
     
     
         24 . The method according to  claim 21 , wherein the solvent is removed from the eluate by rotational distillation, spray drying, thin film drying or freeze drying. 
     
     
         25 . The method according to  claim 24 , wherein the solvent is removed from the eluate by thin film drying.