US2014377265A1PendingUtilityA1

Anticoagulant antidotes

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Assignee: VAN RYN JOANNEPriority: Mar 30, 2011Filed: Jul 22, 2014Published: Dec 25, 2014
Est. expiryMar 30, 2031(~4.7 yrs left)· nominal 20-yr term from priority
Inventors:Joanne Van RynKeith CanadaRobert CopenhaverNorbert HauelTobias LitzenburgerChristopher Ronald SarkoSanjaya SinghAlisa K. Waterman
A61P 39/00A61P 39/02A61P 7/04A61P 7/02C07K 2317/56C07K 2317/55C07K 2317/14C07K 2317/76C07K 2317/21A61K 31/4439C07K 2299/00A61K 39/395A61K 2039/505A61K 39/39583C07K 2317/24C07K 16/44C07K 2317/92C07K 2317/565
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Claims

Abstract

The present invention relates to antibody molecules against anticoagulants, in particular dabigatran, and their use as antidotes of such anticoagulants.

Claims

exact text as granted — not AI-modified
1 . An antibody molecule against dabigatran comprising a heavy chain variable domain with a CDR1 selected from the group consisting of SEQ ID NO: 1, 7, 13, 19, 25, 31, 37, 43, 49, 55, and 61, a CDR2 selected from the group consisting of SEQ ID NO: 2, 8, 14, 20, 26, 32, 38, 44, 50, 56, and 62, and a CDR3 selected from the group consisting of SEQ ID NO: 3, 15, 21, 27, 33, 39, 45, 51, 57, and 63, and a light chain variable domain with a CDR1 selected from the group consisting of SEQ ID NO: 4, 10, 16, 22, 28, 34, 40, 46, 52, and 58, a CDR2 selected from the group consisting of SEQ ID NO: 5, 11, 17, 23, 29, 35, 41, 47, 53, and 59, and a CDR3 selected from the group consisting of SEQ ID NO: 6, 12, 18, 24, 30, 36, 42, 48, 54, 60, and 66. 
     
     
         2 . The antibody molecule of  claim 1  comprising a heavy chain variable domain with a CDR1 of SEQ ID NO: 1, a CDR2 of SEQ ID NO: 2, and a CDR3 of SEQ ID NO: 3, and a light chain variable domain with a CDR1 of SEQ ID NO: 4, a CDR2 of SEQ ID NO: 5, and a CDR3 of SEQ ID NO: 6. 
     
     
         3 . The antibody molecule of  claim 1  comprising a heavy chain variable domain with a CDR1 of SEQ ID NO: 7, a CDR2 of SEQ ID NO: 8, and a CDR3 of SEQ ID NO: 9, and a light chain variable domain with a CDR1 of SEQ ID NO: 10, a CDR2 of SEQ ID NO: 11, and a CDR3 of SEQ ID NO: 12. 
     
     
         4 . The antibody molecule of  claim 1  comprising a heavy chain variable domain with a CDR1 of SEQ ID NO: 13, a CDR2 of SEQ ID NO: 14, and a CDR3 of SEQ ID NO: 15, and a light chain variable domain with a CDR1 of SEQ ID NO: 16, a CDR2 of SEQ ID NO: 17, and a CDR3 of SEQ ID NO: 18. 
     
     
         5 . The antibody molecule of  claim 1  comprising a heavy chain variable domain with a CDR1 of SEQ ID NO: 19, a CDR2 of SEQ ID NO: 20, and a CDR3 of SEQ ID NO: 21, and a light chain variable domain with a CDR1 of SEQ ID NO: 22, a CDR2 of SEQ ID NO: 23, and a CDR3 of SEQ ID NO: 24. 
     
     
         6 . The antibody molecule of  claim 1  comprising a heavy chain variable domain with a CDR1 of SEQ ID NO: 25, a CDR2 of SEQ ID NO: 26, and a CDR3 of SEQ ID NO: 27, and a light chain variable domain with a CDR1 of SEQ ID NO: 28, a CDR2 of SEQ ID NO: 29, and a CDR3 of SEQ ID NO: 30. 
     
     
         7 . The antibody molecule of  claim 1  comprising a heavy chain variable domain with a CDR1 of SEQ ID NO: 31, a CDR2 of SEQ ID NO: 32, and a CDR3 of SEQ ID NO: 33, and a light chain variable domain with a CDR1 of SEQ ID NO: 34, a CDR2 of SEQ ID NO: 35, and a CDR3 of SEQ ID NO: 36. 
     
     
         8 . The antibody molecule of  claim 1  comprising a heavy chain variable domain with a CDR1 of SEQ ID NO: 37, a CDR2 of SEQ ID NO: 38, and a CDR3 of SEQ ID NO: 39, and a light chain variable domain with a CDR1 of SEQ ID NO: 40, a CDR2 of SEQ ID NO: 41, and a CDR3 of SEQ ID NO: 42. 
     
     
         9 . The antibody molecule of  claim 1  comprising a heavy chain variable domain with a CDR1 of SEQ ID NO: 43, a CDR2 of SEQ ID NO: 44, and a CDR3 of SEQ ID NO: 45, and a light chain variable domain with a CDR1 of SEQ ID NO: 46, a CDR2 of SEQ ID NO: 47, and a CDR3 of SEQ ID NO: 48. 
     
     
         10 . The antibody molecule of  claim 1  comprising a heavy chain variable domain with a CDR1 of SEQ ID NO: 49, a CDR2 of SEQ ID NO: 50, and a CDR3 of SEQ ID NO: 51, and a light chain variable domain with a CDR1 of SEQ ID NO: 52, a CDR2 of SEQ ID NO: 53, and a CDR3 of SEQ ID NO: 54. 
     
     
         11 . The antibody molecule of  claim 1  comprising a heavy chain variable domain with a CDR1 of SEQ ID NO: 55, a CDR2 of SEQ ID NO: 56, and a CDR3 of SEQ ID NO: 57, and a light chain variable domain with a CDR1 of SEQ ID NO: 58, a CDR2 of SEQ ID NO: 59, and a CDR3 of SEQ ID NO: 60. 
     
     
         12 . The antibody molecule of  claim 1  comprising a heavy chain variable domain with a CDR1 of SEQ ID NO: 61, a CDR2 of SEQ ID NO: 62, and a CDR3 of SEQ ID NO: 63, and a light chain variable domain with a CDR1 of SEQ ID NO: 64, a CDR2 of SEQ ID NO: 65, and a CDR3 of SEQ ID NO: 66. 
     
     
         13 . (canceled) 
     
     
         14 . The antibody molecule of  claim 1  comprising a heavy chain variable domain of SEQ ID NO: 70, and a light chain variable domain of SEQ ID No: 71. 
     
     
         15 . The antibody molecule of  claim 1  comprising a heavy chain variable domain of SEQ ID NO: 72, and a light chain variable domain of SEQ ID No: 73. 
     
     
         16 . The antibody molecule of  claim 1  comprising a heavy chain variable domain of SEQ ID NO: 74, and a light chain variable domain of SEQ ID No: 75. 
     
     
         17 . The antibody molecule of  claim 1  comprising a heavy chain variable domain of SEQ ID NO: 76, and a light chain variable domain of SEQ ID No: 77. 
     
     
         18 . The antibody molecule of  claim 1  comprising a heavy chain variable domain of SEQ ID NO: 78, and a light chain variable domain of SEQ ID No: 79. 
     
     
         19 . The antibody molecule of  claim 1  comprising a heavy chain variable domain of SEQ ID NO: 80, and a light chain variable domain of SEQ ID No: 81. 
     
     
         20 . The antibody molecule of  claim 1  comprising a heavy chain variable domain of SEQ ID NO: 82, and a light chain variable domain of SEQ ID No: 83. 
     
     
         21 . The antibody molecule of  claim 1  comprising a heavy chain variable domain of SEQ ID NO: 84, and a light chain variable domain of SEQ ID No: 85. 
     
     
         22 . The antibody molecule of  claim 1  comprising a heavy chain variable domain of SEQ ID NO: 86, and a light chain variable domain of SEQ ID No: 87. 
     
     
         23 . The antibody molecule of  claim 1  comprising a heavy chain variable domain of SEQ ID NO: 88, and a light chain variable domain of SEQ ID No: 89. 
     
     
         24 . The antibody molecule of  claim 1  comprising a heavy chain variable domain of SEQ ID NO: 90, and a light chain variable domain of SEQ ID No: 91. 
     
     
         25 . (canceled) 
     
     
         26 . (canceled) 
     
     
         27 . The antibody molecule of  claim 1 , wherein the light chain variable domain is fused to a constant domain of SEQ ID NO: 97. 
     
     
         28 . The antibody molecule of  claim 1 , wherein the heavy chain variable domain is fused to a constant domain of SEQ ID NO: 98. 
     
     
         29 . The antibody molecule of  claim 1  comprising a heavy chain of SEQ ID NO: 95, and a light chain of SEQ ID No: 96. 
     
     
         30 . (canceled) 
     
     
         31 . (canceled) 
     
     
         32 . The antibody molecule of  claim 1 , wherein the antibody is a polyclonal antibody, a monoclonal antibody, a human antibody, a humanized antibody, a chimeric antibody, a fragment of an antibody, in particular a Fab, Fab′, or F(ab′) 2  fragment, a single chain antibody, in particular a single chain variable fragment (scFv), a Small Modular Immunopharmaceutical (SMIP), a domain antibody, a nanobody, a diabody, or a Designed Ankyrin Repeat Protein (DARPin). 
     
     
         33 . The antibody molecule of  claim 1  for use in medicine. 
     
     
         34 . Antibody molecule of  claim 1  for use in the therapy or prevention of side effects of anticoagulant therapy, and/or for reversal of an overdosing of an anticoagulant. 
     
     
         35 . Antibody molecule of  claim 34 , wherein the side effect is a bleeding event. 
     
     
         36 . Method of treatment or prevention of side effects of anticoagulant therapy, or of an overdosing event in anticoagulant therapy, comprising administering an effective amount of an antibody molecule of  claim 1  or  32  to a patient in need thereof. 
     
     
         37 . Method of manufacturing an antibody molecule of  claim 1  or  32 , comprising
 (a) providing a host cell comprising one or more nucleic acids encoding said antibody molecule in functional association with an expression control sequence, 
 (b) cultivating said host cell, and 
 (c) recovering the antibody molecule from the cell culture. 
 
     
     
         38 . A kit comprising an antibody of  claim 1  or  32 , or a pharmaceutical composition thereof. 
     
     
         39 . A kit comprising:
 (a) an antibody of  claim 1  or  32 , or a pharmaceutical composition thereof;   (b) a container; and   (c) a label.   
     
     
         40 . A kit comprising an antibody of  claim 1  or  32 , and dabigatran, dabigatran etexilate, a prodrug of dabigatran or a pharmaceutically acceptable salt thereof. 
     
     
         41 . A method for neutralizing or partially neutralizing dabigatran or 1-O-acylglucuronide of dabigatran in a patient being treated with dabigatran, dabigatran etexilate, a prodrug of dabigatran or a pharmaceutically acceptable salt thereof, comprising administering an antibody of  claim 1  or  32 , or a pharmaceutical composition thereof. 
     
     
         42 . A method for neutralizing or partially neutralizing dabigatran or 1-O-acylglucuronide of dabigatran in a patient comprising:
 (a) confirming that a patient was being treated with dabigatran, dabigatran etexilate, a prodrug of dabigatran or a pharmaceutically acceptable salt thereof, and the amount that was taken by the patient;   (b) neutralizing dabigatran or 1-O-acylglucuronide with an antibody of  claim 1  or  32  prior to performing a clotting or coagulation test or assay wherein dabigatran or the 1-O-acylglucuronide of dabigatran would interfere with the accurate read out of the test or assay results;   (c) performing the clotting or coagulation test or assay on a sample taken from the patient to determine the level of clot formation without dabigatran or 1-O-acylglucuronide of dabigatran present; and   (d) adjusting an amount of dabigatran, dabigatran etexilate, a prodrug of dabigatran or a pharmaceutically acceptable salt thereof administered to the patient in order to achieve the appropriate balance between clot formation and degradation in a patient.   
     
     
         43 . A method for reducing the concentration of dabigatran or 1-O-acylglucuronide of dabigatran in plasma of a patient being treated with dabigatran, dabigatran etexilate, a prodrug of dabigatran or a pharmaceutically acceptable salt thereof, comprising the step of administering an antibody of  claim 1  or  32  or pharmaceutical composition thereof that neutralizes the activity of dabigatran or 1-O-acylglucuronide in the patient. 
     
     
         44 . A method of reversal of the anticoagulant effect of dabigatran or 1-O-acylglucuronide of dabigatran in a patient being treated with dabigatran, dabigatran etexilate, a prodrug of dabigatran or a pharmaceutically acceptable salt thereof, wherein the patient either has major bleeding considered life-threatening or leading to hemodynamic compromise, or wherein the patient requires emergency medical procedures, comprising the step of administering an antibody of  claim 1  or  32  or pharmaceutical composition thereof that neutralizes the activity of dabigatran or 1-O-acylglucuronide in the patient. 
     
     
         45 . A method for reversing or reducing the activity of dabigatran or 1-O-acylglucuronide of dabigatran in a patient experiencing bleeding or at risk for bleeding due to an impaired clotting ability or trauma, comprising the steps of:
 (d) determining the amount of dabigatran or 1-O-acylglucuronide of dabigatran present in the patient;   (e) administering an effective amount of an antibody of  claim 1  or  32  or pharmaceutical composition thereof to reverse or reduce the activity of dabigatran or 1-O-acylglucuronide of dabigatran determined in the patient; and   (f) monitoring a thrombin clotting time of the patient to ensure a reversal or reduction in activity of dabigatran or 1-O-acylglucuronide of dabigatran has been reached.   
     
     
         46 . (canceled) 
     
     
         47 . (canceled) 
     
     
         48 . (canceled)

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