Dual Variable Domain Immunoglobulin and Uses Thereof

Abstract

The present invention relates to engineered multivalent and multispecific binding proteins, methods of making, and specifically to their uses in the prevention and/or treatment of acute and chronic inflammatory and other diseases.

1 - 51 . (canceled) 
     
     
         52 . An isolated nucleic acid encoding an amino acid sequence of at least one of two polypeptide chains that form a binding protein, wherein said at least one polypeptide chain is:
 (a) a first polypeptide chain of said binding protein that comprises formula 1: VD1-(X1)n-VD2-C-(X2)n, wherein VD1 is a first heavy chain variable domain, VD2 is a second heavy chain variable domain, C is a heavy chain constant domain, X1 is a linker with the proviso that it is not CH1, X2 is an Fc region, and n is 0 or 1; or   (b) a second polypeptide chain of said binding protein that comprises formula 2: VD1-(X1)n-VD2-C-(X2)n, wherein VD1 is a first light chain variable domain, VD2 is a second light chain variable domain, C is a light chain constant domain, X1 is a linker with the proviso that it is not CH1, X2 does not comprise an Fc region, and n is 0 or 1;   wherein said first polypeptide chain and said second polypeptide chain of said binding protein form two antigen binding sites.   
     
     
         53 . A vector comprising an isolated nucleic acid according to  claim 52 . 
     
     
         54 . The vector of  claim 53  wherein said vector is selected from the group consisting of pcDNA, pTT, pTT3, pEFBOS, pBV, pJV, pcDNA3.1 TOPO, pEF6 TOPO, and pBJ. 
     
     
         55 . A host cell comprising a vector according to  claim 53 . 
     
     
         56 . The host cell according to  claim 55 , wherein said host cell is a prokaryotic cell. 
     
     
         57 . The host cell according to  claim 56 , wherein said host cell is  E. coli.    
     
     
         58 . The host cell according to  claim 55 , wherein said host cell is a eukaryotic cell. 
     
     
         59 . The host cell according to  claim 58 , wherein said eukaryotic cell is selected from the group consisting of protist cell, animal cell, plant cell, and fungal cell. 
     
     
         60 . The host cell according to  claim 58 , wherein said eukaryotic cell is an animal cell selected from the group consisting of: a mammalian cell, an avian cell, and an insect cell. 
     
     
         61 . The host cell according to  claim 58 , wherein said host cell is a CHO cell. 
     
     
         62 . The host cell according to  claim 58 , wherein said host cell is a COS cell. 
     
     
         63 . The host cell according to  claim 58 , wherein said host cell is a yeast cell. 
     
     
         64 . The host cell according to  claim 63 , wherein said yeast cell is  Saccharomyces cerevisiae.    
     
     
         65 . The host cell according to  claim 58 , wherein said host cell is an insect Sf9 cell. 
     
     
         66 - 76 . (canceled)