Cell cultivation method and cell culture

Abstract

Provided is a cell cultivation method in which the cell is cultured using a peptide hydrogel as a scaffold, for carrying out high-dimensional culture of a cell such as porcine hepatocyte, human hepatocyte, porcine pancreatic islet or human pancreatic islet for a long period under conditions where cell survival, cell morphology and cell functions are maintained. Also provided are a cell culture including a cell and a peptide hydrogel obtained by the above-described cultivation method, a bioreactor including the cell culture, and a cell preparation including the cell culture.

1 - 12 . (canceled) 
     
     
         13 . A method for culturing a primary cell in a cell culture, comprising:
 providing differentiated functional primary cells selected from the group consisting of porcine hepatocytes and human hepatocytes; and   culturing the differentiated functional primary cells using an insert and a sell-assembling peptide hydrogel of SEQ ID NO:1.   
     
     
         14 . The method of claim  1 , further comprising forming at least one selected from cell adhesion apparatus and bile canaliculi. 
     
     
         15 . The method of claim  1 , wherein during the culturing step the differentiated functional primary cells form a spheroidal morphology.