Chemiluminescence sensor array

Abstract

Embodiments of the invention relate to integrated chemiluminescence devices and methods for monitoring molecular binding utilizing these devices and methods. These devices and methods can be used, for example, to identify antigen binding to antibodies. The devices include both a chemiluminescence material and a detector integrated together.

1 - 21 . (canceled) 
     
     
         22 . A method comprising:
 introducing an antigen to a device, the device comprising a chemiluminescent material, an antibody and a light detector,   a filter between the chemiluminescent material and the light detector;   wherein the chemiluminescent material luminesces upon immediate binding between the antigen and the antibody.   
     
     
         23 . The method of  claim 22 , wherein the device further comprises a support substrate and the chemiluminescent material, the antibody, and the light detector are on the support substrate. 
     
     
         24 . The method of  claim 23 , wherein the support substrate comprises glass, nickel, magnetic metal, gold, silicon, nitrocellulose, or polyvinylidene difluoride (PVDF). 
     
     
         25 . The method of  claim 22 , wherein the antibody comprises a conjugate enzyme for activating the chemiluminescent material. 
     
     
         26 . The method of  claim 25 , wherein the conjugate enzyme is horseradish peroxidase. 
     
     
         27 . The method of  claim 22 , wherein the antibody is capable of activating the chemiluminescent material upon binding to the antigen. 
     
     
         28 . The method of  claim 22 , wherein the chemiluminescent material comprises a cyclic diacylhydrazides. 
     
     
         29 . The method of  claim 22 , wherein the chemiluminescent material comprises a material selected from the group consisting of luminol. N-(4-An1inobutyl)-N-ethylisoluminol, 4-Aminophthalhydrazide monohydrate, Bis(2-carbopentyloxy-3,5,6-trichlorophenyl)oxalate, 9,10-Bis(phenylethynyl)anthracene,5,12-Bis(phenylethynyl)naphthacene, 2-Chloro-9,10-bis(phenyletl1ynyl)anthracene, 1,8-Dichloro-9,10-bis(phenylethynyl)anthracene, Lucifer Yellow CH dipotassium salt, Lucifer yellow VS dilithium salt, 85% (dye content), 2,4,5-Triphenylimidazole, 9,10-Diphenylanthracene, Rubrene, and Tetrakis(dimethylamino)ethylene. 
     
     
         30 . The method of  claim 22 , wherein the light detector is a charge-coupled device (CCD) or a complimentary metal-oxide semiconductor (CMOS). 
     
     
         31 . The method of  claim 22 , wherein a metal-nanoparticle tethered antibody also capable of specifically binding to the antigen is applied after the antigen is bound to the antibody. 
     
     
         32 . A method of making an integrated device comprising:
 attaching a light detector to a support comprising a chemiluminescent material and a biopolymer.   
     
     
         33 . The method of  claim 32 , wherein the biopolymer is an antigen. 
     
     
         34 . The method of  claim 32 , wherein the support substrate comprises glass, nickel, magnetic metal, gold, silicon, nitrocellulose, or polyvinylidene difluoride (PVDF). 
     
     
         35 . The method of  claim 33 , wherein the antibody comprises a conjugate enzyme for activating the chemiluminescent material. 
     
     
         36 . The method of  claim 35 , wherein the conjugate enzyme is horseradish peroxidase. 
     
     
         37 . The method of  claim 33 , wherein the antibody is capable of activating the chemiluminescent material upon binding to an antigen. 
     
     
         38 . The method of  claim 33 , wherein the antibody is configured to bind to an antigen of interest. 
     
     
         39 . The method of  claim 32 , wherein the chemiluminescent material comprises a cyclic diacylhydrazides. 
     
     
         40 . The method of  claim 32 , wherein the chemiluminescent material comprises a material selected from the group consisting of luminol, N-(4-Aminobutyl)-N-ethylisoluminol, 4-Aminophthalhydrazide monohydrate, Bis(2-carbopentyloxy-3,5,6-trichlorophenyl)oxalate, 9,29-Bis(phenylethynyl)anthracene, 5,12-Bis(phenylethynyl)naphthacene, 2-Chloro-9,29-bis(phenylethynyl)anthracene, 1,8-Dichloro-9,29-bis(phenylethynyl)anthracene Lucifer Yellow CH dipotassium salt, Lucifer yellow VS dilithium salt, 85% (dye content), 2,4,5-Triphenylimidazole, 9,29-Diphenylanthracene, Rubrene, and Tetrakis(dimethylamino)ethylene. 
     
     
         41 . The method of  claim 32 , wherein the light detector is a charge-coupled device (CCD) or a complimentary metal-oxide semiconductor (CMOS). 
     
     
         42 - 43 . (canceled) 
     
     
         44 . The method of  claim 22 , wherein the antibody is configured to activate the chemiluminescent material. 
     
     
         45 . The method of  claim 22 , wherein the light detector is configured to detect an activity of the chemiluminescent material.