Rapid Preparation of Stem Cell Matrices for Use in Tissue and Organ Treatment and Repair

Abstract

A rapid method for preparing stem cell and physiologically acceptable matrix compositions for use in tissue and organ repair is described. Compared with previous tissue engineering materials, the stem cell-matrix compositions of the present invention do not require long-term incubation or cultivation in vitro prior to use in in vivo applications. The stem cells can be from numerous sources and may be homogeneous, heterogeneous, autologous, and/or allogeneic in the matrix material. The stem cell-matrix compositions provide point of service utility for the practitioner, wherein the stem cells and matrix can be combined not long before use, thereby alleviating costly and lengthy manufacturing procedures. In addition, the stem cells offer unique structural properties to the matrix composition which improves outcome and healing after use. Use of stem cells obtained from muscle affords contractility to the matrix composition.

1 .- 27 . (canceled) 
     
     
         28 . A method of preparing a vessel like lumen comprising seeding a single layer of small intestine submucosa (SIS) with muscle-derived progenitor cells (MSC) to form a MSC/SIS matrix; folding the MSC/SIS matrix into a vessel-like lumen, thereby preparing a vessel like lumen. 
     
     
         29 . The method of  claim 28 , wherein the MSC are inoculated onto SIS simultaneously with fibrin glue to form the MSC/SIS matrix. 
     
     
         30 . The method of  claim 28 , wherein the MSC are autologous or allogeneic. 
     
     
         31 . The method of  claim 30 , wherein allogeneic cells are from a donor with class I and class II histocompatibility antigen closest matched with a recipient. 
     
     
         32 . The method of  claim 31 , wherein the recipient is on immunosuppressive or immunomodulatory therapy started before, during, and/or after the matrix is applied or introduced into the recipient. 
     
     
         33 . The method of  claim 32 , wherein the immunosuppressive drug is cyclosporine A. 
     
     
         34 . A method of repairing a blood vessel comprising introducing to a subject in need thereof a vessel like lumen prepared by the method of  claim 28 . 
     
     
         35 . The method of  claim 28 , wherein the MSC/SIS matrix is formed by a method comprising:
 a) admixing a preparation comprising the MSC with the SIS to form a cell matrix dressing; and   b) incubating the stem cell matrix dressing in vitro for 5 seconds to about 12 hours prior to folding into a vessel-like lumen.   
     
     
         36 . A method of altering or improving biomechanical properties of small intestine submucosa (SIS), the method comprising seeding one or more layers of small intestine submucosa (SIS) with muscle-derived progenitor cells (MSC) to form a MSC/SIS matrix, wherein the MSC/SIS has a decreased horizontal/cross sectional elasticity versus the longitudinal direction compared to a SIS not seeded with MSC. 
     
     
         37 . The method of  claim 36 , wherein the MSC/SIS matrix has an increased elasticity in the horizontal/cross sectional direction compared to the SIS not seeded with MSC. 
     
     
         38 . The method of  claim 36 , the altering or improving biomechanical properties is assessed by biaxial mechanical testing. 
     
     
         39 . The method of  claim 36 , wherein the MSC/SIS matrix has a curved shape. 
     
     
         40 . The method of  claim 39 , wherein the MSC/SIS matrix is contractible. 
     
     
         41 . The method of  claim 40 , wherein the MSC/SIS matrix has a tonic contractile activity. 
     
     
         42 . The method of  claim 36 , wherein the MSC are inoculated onto SIS simultaneously with fibrin glue to form the MSC/SIS matrix. 
     
     
         43 . The method of  claim 36 , wherein the MSC are autologous or allogeneic.