Methods of therapeutic treatment of eyes

Abstract

Provided are electrokinetically-altered aqueous fluids (e.g., gas-enriched electrokinetic fluids) comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures in an amount sufficient to provide modulation of at least one of cellular membrane potential and cellular membrane conductivity, and therapeutic compositions and methods for use in treating an irritation, infection or inflammatory eye condition, comprising administering to, by contacting the eye of a subject in need thereof a therapeutically effective amount of an electrokinetically-altered aqueous fluid. The electrokinetically-altered fluids or therapeutic compositions and methods include electrokinetically-altered ioinic aqueous fluids optionally in combination with other therapeutic agents. Other embodiments include particular routes of administration or formulations for the electrokinetically-altered fluids (e.g., electrokinetically-altered gas-enriched fluids) and therapeutic compositions for use in treating eye conditions. Certain embodiments relate to cosmetic and/or therapeutic fluids and/or methods of treatment utilizing the fluids to treat a cosmetic and/or therapeutic symptom related to eye conditions and/or diseases.

1 .- 69 . (canceled) 
     
     
         70 . A method of harvesting, and/or storing and/or transporting organs, organ samples, living tissues or cells, comprising contacting organ, organ sample, living tissue or cells, during harvesting, storing and/or transporting thereof, with an effective amount of an ionic aqueous solution of charge-stabilized oxygen-containing nanobubbles having an average diameter of less than 100 nanometers persisting in the ionic aqueous fluid sufficient for preserving, storing and/or transplanting, with less cell damage, the organ, organ sample, living tissue or cells, during the harvesting, storing, transporting and/or transplanting thereof. 
     
     
         71 . The method of  claim 70 , wherein contacting the organ, organ sample, living tissue or cells, during harvesting, storing, transporting and/or transplanting thereof with the ionic aqueous solution comprises perfusing the organ, organ sample, living tissue or cells with the ionic aqueous solution. 
     
     
         72 . The method of  claim 70 , wherein the organ, organ sample, living tissue or cells comprises at least one of liver, kidney, heart, eye, hand, foot, brain and stem cells. 
     
     
         73 . The method of  claim 70 , wherein oxygen is present in the ionic aqueous solution in an amount selected from the group consisting of at least 15 ppm, at least 25 ppm, at least 30 ppm, at least 40 ppm, at least 50 ppm, or at least 60 ppm oxygen at atmospheric pressure. 
     
     
         74 . The method of  claim 70 , wherein the amount of charge-stabilized oxygen-containing nanobubbles in the ionic aqueous solution is at least 15 ppm at atmospheric pressure. 
     
     
         75 . The method of  claim 73 , wherein at least 90% of oxygen present in the ionic aqueous solution is in the charge-stabilized oxygen-containing nanobubbles. 
     
     
         76 . The method of  claim 70 , wherein the ionic aqueous solution comprises at least one of solvated electrons stabilized by molecular oxygen, and electrokinetically modified or charged oxygen species. 
     
     
         77 . The method of  claim 70 , wherein the ionic aqueous solution comprises saline or oxygen-enriched saline. 
     
     
         78 . The method of  claim 70 , wherein the ionic aqueous solution further comprises at least one therapeutic agent selected from the group consisting of: anti-microbial agent, anti-inflammatory agent, pain reliever, anesthetic, vitamin, cytokine, adjuvant, preservative, salt, and combinations thereof. 
     
     
         79 . A method of providing artificial blood to an organ, organ sample, living tissue or cells, comprising contacting an organ, organ sample, living tissue or cells with an effective amount of an ionic aqueous solution of charge-stabilized oxygen-containing nanobubbles having an average diameter of less than 100 nanometers persisting in the ionic aqueous fluid sufficient for preserving, with less cell damage, the organ, organ sample, living tissue or cells. 
     
     
         80 . The method of  claim 79 , wherein contacting the organ, organ sample, living tissue or cells with the ionic aqueous solution comprises perfusing the organ, organ sample, living tissue or cells with the ionic aqueous solution. 
     
     
         81 . The method of  claim 79 , wherein the organ, organ sample, living tissue or cells comprises at least one of liver, kidney, heart, eye, hand, foot, brain and stem cells. 
     
     
         82 . The method of  claim 79 , wherein oxygen is present in the ionic aqueous solution in an amount selected from the group consisting of at least 15 ppm, at least 25 ppm, at least 30 ppm, at least 40 ppm, at least 50 ppm, or at least 60 ppm oxygen at atmospheric pressure. 
     
     
         83 . The method of  claim 79 , wherein the amount of charge-stabilized oxygen-containing nanobubbles in the ionic aqueous solution is at least 15 ppm at atmospheric pressure. 
     
     
         84 . The method of  claim 82 , wherein at least 90% of oxygen present in the ionic aqueous solution is in the charge-stabilized oxygen-containing nanobubbles. 
     
     
         85 . The method of  claim 79 , wherein the ionic aqueous solution comprises at least one of solvated electrons stabilized by molecular oxygen, and electrokinetically modified or charged oxygen species. 
     
     
         86 . The method of  claim 79 , wherein the ionic aqueous solution comprises saline or oxygen-enriched saline. 
     
     
         87 . The method of  claim 79 , wherein the ionic aqueous solution further comprises at least one therapeutic agent selected from the group consisting of: anti-microbial agent, anti-inflammatory agent, pain reliever, anesthetic, vitamin, cytokine, adjuvant, preservative, salt, and combinations thereof. 
     
     
         88 . The method of  claim 79 , wherein contacting the organ, organ sample, living tissue or cells with the effective amount of the ionic aqueous solution is during a surgical procedure. 
     
     
         89 . The method of  claim 88 , wherein the surgical procedure comprises at least one selected from the group consisting of a coronary surgery, a shock-related procedure, a trauma-related procedure, and an eye-related surgery. 
     
     
         90 . An artificial blood, comprising a physiological saline solution of charge-stabilized oxygen-containing nanobubbles having an average diameter of less than 100 nanometers persisting in the solution sufficient for preserving, with less cell damage, an organ, organ sample, living tissue or cells. 
     
     
         91 . The method of  claim 90 , wherein oxygen is present in the physiological saline solution in an amount selected from the group consisting of at least 15 ppm, at least 25 ppm, at least 30 ppm, at least 40 ppm, at least 50 ppm, or at least 60 ppm oxygen at atmospheric pressure. 
     
     
         92 . The method of  claim 90 , wherein the amount of charge-stabilized oxygen-containing nanobubbles in the physiological saline solution is at least 15 ppm at atmospheric pressure. 
     
     
         93 . The method of  claim 90 , wherein the physiological saline solution further comprises at least one therapeutic agent selected from the group consisting of: anti-microbial agent, anti-inflammatory agent, pain reliever, anesthetic, vitamin, cytokine, adjuvant, preservative, salt, and combinations thereof.