Method for producing a biosensor

Abstract

An object of the present invention is to provide a biosensor comprising a hydrogel capable of immobilizing a physiologically active substance thereon, which can be produced conveniently by use of a safe material, and a method for producing the same. The present invention provides a method for producing a biosensor, which comprises bringing a polymer containing an activated carboxyl group into contact with a substrate surface coated with an organic layer having an amino group to thereby bind the polymer to the organic layer.

1 . A method for producing a biosensor, which comprises bringing a polymer containing an activated carboxyl group into contact with a substrate surface coated with an organic layer having an amino group to thereby bind the polymer to the organic layer. 
     
     
         2 . The method according to  claim 1 , wherein the polymer containing an activated carboxyl group is a polymer containing an active esterified carboxyl group. 
     
     
         3 . The method according to  claim 1 , wherein the substrate surface coated with an organic layer having an amino group is a substrate surface coated with alkanethiol having an amino group. 
     
     
         4 . The method according to  claim 1 , wherein the substrate surface coated with an organic layer having an amino group is a substrate surface coated with a mixture of alkanethiol having an amino group and alkanethiol having a hydrophilic group. 
     
     
         5 . The method according to  claim 4 , wherein the hydrophilic group in the alkanethiol having a hydrophilic group is a hydroxyl group or oligoethylene glycol group. 
     
     
         6 . The method according to  claim 5 , wherein the mixture of alkanethiol having an amino group and alkanethiol having a hydrophilic group comprises the alkanethiol having an amino group and the alkanethiol having a hydrophilic group at a molar ratio ranging from 1/1 to 1/1,000,000. 
     
     
         7 . The method according to  claim 4 , wherein the alkanethiol having an amino group has a molecular length larger than that of the alkanethiol having a hydrophilic group. 
     
     
         8 . The method according to  claim 1 , wherein the polymer containing a carboxyl group is polysaccharide. 
     
     
         9 . The method according to  claim 1 , wherein the polymer containing an activated carboxyl group is reacted in the state of a thin film formed on the substrate with the organic layer having an amino group. 
     
     
         10 . The method according to  claim 9 , wherein the thin film is formed on the substrate by a spin coating method or spray coating method. 
     
     
         11 . The method according to  claim 1 , wherein the polymer containing an activated carboxyl group is a polymer which is obtained by activating a polymer containing a carboxyl group with a carbodiimide derivative, a nitrogen-containing compound or a phenol derivative. 
     
     
         12 . The method according to  claim 1 , wherein the polymer containing an activated carboxyl group is a polymer which is obtained by activating a polymer containing a carboxyl group with the following morpholine derivative. 
       
         
           
           
               
               
           
         
       
     
     
         13 . A biosensor which is produced by the method according to  claim 1 . 
     
     
         14 . A method for detecting or measuring a substance interacting with a physiologically active substance, which comprises a step of bringing a biosensor according to  claim 13  having a surface covalently bonded with the physiologically active substance into contact with a test substance. 
     
     
         15 . The method according to  claim 14 , wherein the substance interacting with a physiologically active substance is detected or measured by a nonelectrochemical method. 
     
     
         16 . The method according to  claim 14 , wherein the substance interacting with a physiologically active substance is detected or measured by surface plasmon resonance analysis.