Collagen and fibrin microthreads in a discrete thread model of in vitro acl scaffold regeneration

Abstract

Compositions that include fibrin microthreads are provided. The compositions can include one or more therapeutic agents including cytokines and interleukins, extracellular matrix proteins and/or biologically active fragments thereof (e.g., RGD-containing peptides), hormones, vitamins, nucleic acids, chemotherapeutics, antibiotics, and cells. Also provided are methods of making compositions that include fibrin microthreads. Also provided are methods for using the compositions to repair or ameliorate damaged or defective organs or tissues.

1 .- 50 . (canceled) 
     
     
         51 . A method of repairing a tissue, comprising suturing a tissue in need of repair with a fibrin microthread to cause tissue repair, wherein the fibrin microthread is formed by a process comprising the steps of:
 combining a first volume of fibrinogen and a second volume of a molecule capable of forming fibrin from the fibrinogen to form a mixture;   transferring the mixture to a lumen containing device;   disposing a distal end of the lumen containing device in an aqueous bath;   moving the distal end of the lumen containing device through the aqueous bath while extruding the mixture from the distal end of the lumen containing device into the aqueous bath; and   incubating the mixture in the aqueous bath for a predetermined incubation time to form the fibrin microthread.   
     
     
         52 . The method of  claim 51 , wherein the fibrin microthread is associated with one or more of a substrate or a braided yarn or other hierarchically organized rope, a woven or non-woven mesh, a surgical needle, a surgical pin, a surgical screw, a surgical plate, a physiologically acceptable patch, dressing, bandage, or a natural or mechanical valve. 
     
     
         53 . The method of  claim 51 , wherein the tissue in need of repair is selected from epithelial tissue, skin, muscle, and a connective tissue. 
     
     
         54 . The method of  claim 53 , wherein the tissue in need of repair is skin. 
     
     
         55 . The method of  claim 51 , wherein the tissue in need of repair comprises a traumatic injury, a congenital malformation, a malfunction, or malformation resulting from an infection, or malformation resulting from a surgical procedure. 
     
     
         56 . The method of  claim 51 , wherein the tissue repair is tissue augmentation. 
     
     
         57 . The method of  claim 51 , wherein the tissue repair is skin grafting. 
     
     
         58 . The method of  claim 51 , wherein the fibrinogen is human fibrinogen or fibrinogen of a non-human primate, a domesticated animal, or a rodent generated either naturally or through recombinant or synthetic protein production techniques. 
     
     
         59 . The method of  claim 51 , wherein the wherein the molecule capable of forming fibrin is a protease. 
     
     
         60 . The method of  claim 59 , wherein the wherein the protease is thrombin.