Levetiracetam Immunoassays

Abstract

Methods, compositions and kits are disclosed directed at levetiracetam derivatives, immunogens, signal generating moieties, antibodies that bind levetiracetam and immunoassays for detection of levetiracetam.

1 - 47 . (canceled) 
     
     
         48 . A compound of the formula: 
       
         
           
           
               
               
           
         
         wherein R 2  is —Y—Z, wherein Y is a linking group selected from the group consisting of —(CH 2 )n—C(O)—, —C(O)(CH 2 )n—, —C(O)(CH 2 )n-NHC(O)—, —C(O)(CH 2 )n-NHC(O)(CH 2 )n—, —(CH 2 )nSCH 2 C(O)—, —(CH 2 )n—C(O)NH—(CH 2 )n—, —(CH 2 )n-NH—C(O)—, —(CH 2 )n-NH—C(O)—(CH 2 )n—, —C(O)—(CH 2 )n—, and —(CH 2 )n-NH—; and n is an integer from 1 to 10; and 
         Z is selected from the group consisting of hydrogen, —OH, —SH, —S-acyl; —O-lower alkyl, halogen, —NH 2 , epoxy, maleimidyl, haloacetamide, carboxyl, activated carboxyl, hydroxysuccinimidyl, succiminidyl, carbonate, anhydride, imidate, an immunogenic carrier, a protein and a label; and 
         acid salts thereof. 
       
     
     
         49 . The compound of  claim 48 , wherein the linking group comprises 1-15 carbon atoms and/or 0-6 heteroatoms. 
     
     
         50 . The compound of  claim 48 , wherein the linking group comprises 10-15 carbon atoms and/or 0-6 heteroatoms. 
     
     
         51 . The compound of  claim 48 , wherein Z is selected from the group consisting of an immunogenic carrier, a protein, and a label. 
     
     
         52 . The compound of  claim 48 , wherein the immunogenic carrier is BSA or KLH. 
     
     
         53 . The compound of  claim 48 , wherein the immunogenic carrier is selected from the group consisting of hemocyanins, globulins, albumins, and polysaccharides. 
     
     
         54 . The compound of  claim 48 , wherein Z is a label. 
     
     
         55 . The compound of  claim 54 , wherein the label is an enzyme. 
     
     
         56 . The compound of  claim 55 , wherein the enzyme is G6PDH. 
     
     
         57 . The compound of  claim 55 , wherein the enzyme is selected from alkaline phosphatase, β-galactosidase, and horse radish peroxidase. 
     
     
         58 . The compound of  claim 48 , wherein Z is selected from the group consisting of —SH, halogen, —NH 2 , maleimidyl, carboxyl, and activated carboxyl; and Y is —(CH 2 )n-NH—C(O)—(CH 2 )n- where each n is an integer from one to ten. 
     
     
         59 . A kit for determining an amount of levetiracetam in a medium, said kit comprising
 a) an antibody that binds an epitope present in levetiracetam and a compound of  claim 48 ; and   b) a compound of  claim 48 , wherein Z is a label and said label is an enzyme.   
     
     
         60 . The kit of  claim 59 , wherein the enzyme is G6PDH. 
     
     
         61 . A reaction mixture comprising
 a) a sample suspected of containing levetriacetam;   b) an antibody that binds an epitope present in levetiracetam and a compound of  claim 48 ; and   c) a compound of  claim 48 , wherein Z is a label and said label is an enzyme.   
     
     
         62 . The reaction mixture of  claim 61 , wherein the enzyme is G6PDH. 
     
     
         63 . A method for detecting levetiracetam, said method comprising:
 combining in a reaction mixture: (a) a sample suspected of containing levetiracetam; (b) an antibody that binds an epitope present in levetiracetam and a compound of  claim 48 ; and (c) a compound of  claim 48 , wherein Z is a label;   allowing one or more of the levitiracetam in the sample and the compound of  claim 48 , wherein Z is the label, to bind with the antibody; and   measuring the amount of the compound of  claim 48 , wherein Z is the label, in the mixture that is bound or unbound to the antibody, whereby the presence of levitiracetam in the sample can be detected.   
     
     
         64 . The method of  claim 63 , wherein the label is an enzyme. 
     
     
         65 . The method of  claim 64 , wherein the enzyme is G6PDH.