USE OF miR-199a-5p, TARGETS AND/OR INHIBITORS THEREOF FOR THE DIAGNOSIS, PROGNOSIS AND TREATMENT OF FIBROPROLIFERATIVE DISORDERS

Abstract

The present invention relates to the use of the miRNA expression profile, particularly of miR-199a-5p, and the target genes regulated thereby for the diagnosis, prognosis and use of miR-199a-5p inhibitors for treating fibroproliferative disorders.

1 . An miR-199a-5p inhibitor configured for preventing and/or treating a fibroproliferative disorder. 
     
     
         2 . An miR-199a-5p inhibitor according to  claim 1 , wherein the fibroproliferative disorder is idiopathic pulmonary fibrosis. 
     
     
         3 . An miR-199a-5p inhibitor configured as a medicinal pro duct. 
     
     
         4 . In vitro diagnostic method for a fibroproliferative disorder in a subject, comprising the following steps:
 (i) quantitative measurement of the level of miRNA expression in a biological sample from said subject;   (ii) comparing the miRNA expression profile of the biological sample from said subject with the same miRNA expression profile of a reference biological sample, said expression profile consisting of mir-146b, miR-34a, miR-21, miR-449a, miR-449b, miR-20a, miR-18a, miR-223, miR-449c, miR-147b, miR-152, miR-181ac, miR-451, miR-351, miR-133ac, miR-214, miR-199a-5p, miR-132, miR-222, miR-342-3p, miR-345-5p and miR-221;   (iii) identifying at least one miRNA within said expression profile for which the level of expression by said biological sample from said subject differs with respect to the level of expression of the same at least one miRNA by said reference sample.   
     
     
         5 . In vitro diagnostic method according to  claim 4 , said method further comprising the following step:
 (iv) identifying at least one target gene for which the level of expression is regulated by the level of said at least one miRNA identified in step (iii);   
     
     
         6 . In vitro diagnostic method according to  claim 4 , wherein said at least one miRNA identified in step (iii) is miR-199a-5p. 
     
     
         7 . In vitro diagnostic method according to  claim 5 , wherein said at least one target gene identified in step (iv) codes for caveolin-1. 
     
     
         8 . Method for identifying a subject liable to develop a fibroproliferative disorder, comprising the following steps:
 (i) quantitative measurement of the level of miR-199a-5p expression in a biological sample from said subject;   (ii) comparing the level of miR-199a-5p expression in said biological sample from said subject with the level of miR-199a-5p expression in a reference biological sample;   (iii) detecting an increase in the level of miR-199a-5p expression.   
     
     
         9 . Method according to  claim 8 , said method further comprising the following step:
 (iv) identifying at least one target gene for which the level of expression is regulated by the level of miR-199a-5p expression identified in step (iii).   
     
     
         10 . Method according to  claim 9 , wherein said at least one target gene identified in step (iv) codes for caveolin-1. 
     
     
         11 . An miR-199a-5p inhibitor configured for stimulating wound repair.