Cosmetic Preparations

Abstract

The invention is directed to methods for the treatment of wounds. Such methods utilize novel compositions, including but not limited to amnion-derived multipotent cells (herein referred to as AMP cells), conditioned media derived therefrom (herein referred to as amnion-derived cellular cytokine suspension or ACCS), cell lysates derived therefrom, cell products derived therefrom, each alone or in combination.

1 - 17 . (canceled) 
     
     
         18 . A cosmetic preparation consisting of:
 a) conditioned medium, wherein the conditioned medium is made by a method comprising the steps of
 i) obtaining a placenta and isolating an amnion from the placenta, 
 ii) enzymatically releasing amnion-derived epithelial cells from the amnion, 
 iii) collecting the released amnion-derived epithelial cells, and 
 iv) culturing the collected amnion-derived epithelial cells of step (c) in basal culture medium that is supplemented with human serum albumin and recombinant human EGF; and 
   b) a gelling agent.   
     
     
         19 . The cosmetic preparation of  claim 18  wherein the gelling agent is selected from the group consisting of xanthan, guar gum, starches, alginates, crosslinked polymeric gelling agents and partially neutralized 2-acrylamido-2-methylpropanesulfonic acid polymers. 
     
     
         20 . The cosmetic preparation of  claim 18  wherein the basal medium is IMDM. 
     
     
         21 . The cosmetic preparation of  claim 18  wherein the IMDM is supplemented with 0.5% human serum albumin. 
     
     
         22 . The cosmetic preparation of  claim 18  wherein the recombinant human EGF is at a concentration of 10 ng/mL of culture medium. 
     
     
         23 . The cosmetic preparation of  claim 18  wherein the substantially purified population of cultured amnion-derived epithelial cells, the basal medium, and all medium supplements are free of xeno-contamination.