Chip for analysis of target substance

Abstract

The present invention provides a chip for analysis of a target substance that is compact and allows analysis of a target substance with less time and effort. The chip for analysis of a target substance includes a first flexible substrate 1 , a second flexible substrate 2 , and a third substrate 3 . A flow channel-forming non-bonded area 11 is formed on a bonding surface of the first flexible substrate 1 and the second flexible substrate 2 in a band-like manner and an extraction chamber-forming non-bonded area 5 having a wider band width than the flow channel-forming non-bonded area 11 is formed at a part of the flow channel-forming non-bonded area 11 . The first flexible substrate 1 includes a through-hole 7 that is in contact with the flow channel-forming non-bonded area 11 , a shutter-forming non-bonded area 12 b is formed on a bonding surface of the second flexible substrate 2 and the third substrate 3 at a far side of the through-hole 7 relative to the extraction chamber-forming non-bonded area 5 such that the shutter-forming non-bonded area 12 b and the flow channel-forming non-bonded area 11 intersect above and below, the first flexible substrate 1 and the second flexible substrate 2 include a pressure supply port 18 b that comes through the substrates so as to be in contact with the shutter-forming non-bonded area 12 b , and a magnetic particle 16 that binds to a target substance is placed above the extraction chamber-forming non-bonded area 5.

1 . A chip for analysis of a target substance comprising:
 a laminate, the laminate comprising:
 a first flexible substrate; 
 a second flexible substrate; and 
 a third substrate, wherein 
   
       a flow channel-forming non-bonded area is formed on a bonding surface of the first flexible substrate and the second flexible substrate in a band-like manner and an extraction chamber-forming non-bonded area having a wider band width than the flow channel-forming non-bonded area is formed at a part of the flow channel-forming non-bonded area, 
       the first flexible substrate comprises a through-hole that is in contact with the flow channel-forming non-bonded area, 
       a shutter-forming non-bonded area is formed on a bonding surface of the second flexible substrate and the third substrate in a band-like manner at a far side of the through-hole relative to the extraction chamber-forming non-bonded area such that the shutter-forming non-bonded area and the flow channel-forming non-bonded area intersect above and below via the second flexible substrate being interposed therebetween, 
       at least one of both of the first flexible substrate and the second flexible substrate and the third substrate comprises a pressure supply port that comes through the substrate(s) so as to be in contact with the shutter-forming non-bonded area, 
       a magnetic particle that binds to a target substance is placed above the extraction chamber-forming non-bonded area, 
       a flow channel and an extraction chamber are formable by supplying pressure from the through-hole to raise a site above the flow channel-forming non-bonded area and a site above the extraction chamber-forming non-bonded area, 
       the flow channel is blockable by supplying pressure from the pressure supply port to raise a site above the shutter-forming non-bonded area, and 
       the target substance that is bound to the magnetic particle is capturable by generating a magnetic field at at least one of an undersurface of the third substrate directly below an end of the extraction chamber at the opposite side of the through-hole and a top surface of the first flexible substrate directly above an end of the extraction chamber at the opposite side of the through-hole. 
     
     
         2 . A chip for analysis of a target substance comprising:
 a laminate, the laminate comprising:
 a first flexible substrate; 
 a second flexible substrate; and 
 a third substrate, wherein 
   
       a flow channel-forming non-bonded area is formed on a bonding surface of the first flexible substrate and the second flexible substrate in a band-like manner and a mixing chamber-forming non-bonded area having a wider band width than the flow channel-forming non-bonded area is formed at a part of the flow channel-forming non-bonded area, 
       the first flexible substrate comprises a through-hole that is in contact with the flow channel-forming non-bonded area, 
       shutter-forming non-bonded areas are formed on a bonding surface of the second flexible substrate and the third substrate in a band-like manner at a near side and a far side of the through-hole relative to the mixing chamber-forming non-bonded area such that the shutter-forming non-bonded areas and the flow channel-forming non-bonded area intersect above and below via the second flexible substrate being interposed therebetween, 
       at least one of both of the first flexible substrate and the second flexible substrate and the third substrate comprises pressure supply ports that come through the substrate(s) so as to be in contact with the shutter-forming non-bonded areas, 
       a flow channel and a mixing chamber are formable by supplying pressure from the through-hole to raise a site above the flow channel-forming non-bonded area and a site above the mixing chamber-forming non-bonded area, 
       the flow channel is blockable by supplying pressure from the pressure supply ports to raise sites above the shutter-forming non-bonded areas, and 
       a target substance and a reagent are mixable in the mixing chamber by applying pressure to a top surface of the first flexible substrate above the mixing chamber to deform the mixing chamber. 
     
     
         3 . A chip for analysis of a target substance comprising:
 a laminate, the laminate comprising:
 a first flexible substrate; 
 a second flexible substrate; and 
 a third substrate, wherein 
   
       a flow channel-forming non-bonded area is formed on a bonding surface of the first flexible substrate and the second flexible substrate in a band-like manner, 
       the first flexible substrate comprises a through-hole that is in contact with the flow channel-forming non-bonded area, 
       a first mixing chamber-forming non-bonded area and a second mixing chamber-forming non-bonded area each having a wider band width than the flow channel-forming non-bonded area are each formed at a part of the flow channel-forming non-bonded area on the bonding surface of the first flexible substrate and the second flexible substrate in this order from the through-hole side, 
       shutter-forming non-bonded areas are formed on a bonding surface of the second flexible substrate and the third substrate in a band-like manner at a near side of the through-hole relative to the first mixing chamber-forming non-bonded area and a far side of the through-hole relative to the second mixing chamber-forming non-bonded area such that the shutter-forming non-bonded areas and the flow channel-forming non-bonded area intersect above and below via the second flexible substrate being interposed therebetween, 
       at least one of both of the first flexible substrate and the second flexible substrate and the third substrate includes pressure supply ports that come through the substrate(s) so as to be in contact with the shutter-forming non-bonded areas, 
       a flow channel, a first mixing chamber, and a second mixing chamber are formable by supplying pressure from the through-hole to raise a site above the flow channel-forming non-bonded area, a site above the first mixing chamber-forming non-bonded area, and a site above the second mixing chamber-forming non-bonded area, 
       the flow channel is blockable by supplying pressure from the pressure supply ports to raise sites above the shutter-forming non-bonded areas, and 
       a target substance and a reagent are mixable by moving them between the first mixing chamber and the second mixing chamber. 
     
     
         4 . The chip according to  claim 3 , wherein
 a shutter-forming non-bonded area is further formed on the bonding surface of the second flexible substrate and the third substrate in a band-like manner at a far side of the through-hole relative to the first mixing chamber-forming non-bonded area such that the shutter-forming non-bonded area and the flow channel-forming non-bonded area intersect above and below via the second flexible substrate being interposed therebetween.   
     
     
         5 . The chip according to any one of  claims 1  to  4 , further comprising:
 an amplification portion. 
 
     
     
         6 . The chip according to  claim 5 , wherein
 the amplification portion comprises a PCR amplification portion.   
     
     
         7 . The chip according to any one of  claims 1  to  6 , further comprising:
 an analysis portion. 
 
     
     
         8 . The chip according to  claim 7 , wherein
 the analysis portion comprises an electrophoresis analysis portion.