COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-HYDROXYMETHYLCYTOSINE (5-hmC)

Abstract

The present invention relates generally to the field of molecular biology. More particularly, it concerns methods and compositions for detecting, evaluating, and/or mapping 5-hydroxymethyl-modified cytosine bases within a nucleic acid molecule.

1 .- 162 . (canceled) 
     
     
         163 . A kit comprising purified β-glucosyltransferase and a modified uridine diphosphoglucose (UDP-Glu) molecule. 
     
     
         164 . The kit of  claim 163 , further comprising one or more nucleic acid buffers. 
     
     
         165 . The kit of  claim 163 , further comprising a polymerase. 
     
     
         166 . The kit of  claim 165 , further comprising a composition comprising nucleotides for use with the polymerase. 
     
     
         167 . The kit of  claim 165 , further comprising a restriction enzyme. 
     
     
         168 .- 171 . (canceled) 
     
     
         172 . The kit of  claim 163 , wherein the modified UDP-Glu molecule comprises a linker, a detectable moiety, an isolation tag, a blocking moiety, or a functional moiety. 
     
     
         173 . A kit comprising purified β-glucosyltransferase and a synthetically modified uridine diphosphoglucose (UDP-Glu) molecule, a polymerase, and one or more nucleic acid buffers. 
     
     
         174 . The kit of  claim 173 , further comprising a composition comprising nucleotides for use with the polymerase. 
     
     
         175 . The kit of  claim 173 , further comprising a restriction enzyme. 
     
     
         176 . The kit of  claim 173 , wherein the synthetically modified UDP-Glu molecule comprises a linker, a detectable moiety, an isolation tag, a blocking moiety, or a functional moiety. 
     
     
         177 . A kit comprising purified β-glucosyltransferase and a synthetically modified uridine diphosphoglucose (UDP-Glu) molecule, a polymerase, and one or more nucleic acid buffers, wherein the synthetically modified UDP-Glu comprises a synthetic linker, a detectable moiety, an isolation tag, a blocking moiety, or a functional moiety.