Therapeutic use of cd31 expressing cells

Abstract

As described below, the present invention features compositions and methods related to the isolation, culture and therapeutic use of CD31-expressing cells.

What we claim: 
     
         1 . A composition comprising cells made by the process of selecting cells from bone marrow or peripheral blood of a subject based on the cellular expression of CD31 providing an isolate cells wherein substantially all cells are CD31 +  and expanding the isolate cells. 
     
     
         2 . The composition of  claim 1 , wherein selecting cells is done by mixing bone marrow or peripheral blood with CD31 antibodies providing antibody bound cells and separating the antibody bound cells based on a physical characteristic of the antibodies. 
     
     
         3 . The composition of  claim 2 , wherein the antibodies comprise a fluorescent molecule and selecting is by fluorescence activated cell sorting (FACS). 
     
     
         4 . The composition of  claim 2 , wherein the antibodies are on a magnetic bead and selecting is by a magnetic column. 
     
     
         5 . The composition of  claim 2 , wherein the subject is a human. 
     
     
         6 . The composition of  claim 1 , wherein the expanding the isolated cells is done in a culture media comprising epidermal growth factor, vascular endothelial growth factor (VEGF), human fibroblast growth factor-basic (FGF-2), insulin-like growth factor-I (IGF-1), or ascorbic acid.