In vivo detection of apoptosis

Abstract

The invention provides methods and products, such as kits, useful for determining the apoptotic state of cells in an organism, comprising detecting the presence or abundance of at least one caspase affinity labeling agent in the cells of an animal into which at least one caspase affinity labeling agent has been introduced, wherein the presence or abundance of the caspase affinity labeling agent correlates with the apoptotic state of the cells.

1 - 5 . (canceled) 
     
     
         6 . An in vivo diagnostic method for determining the presence or absence of a disease characterized by the presence of apoptosis comprising detecting the presence of at least one caspase affinity labeling agent in the cells of an animal into which at least one caspase affinity labeling agent has been introduced, wherein the presence or absence of at least one caspase affinity labeling agent correlates with the presence or absence of the disease;
 wherein the caspase affinity labeling agent is a compound of formula I:
   L 1 -A 1 -X 1 —NH—CH(R 1 ′)C(═O)CH 2 F  (I)
 
   
       wherein:
 L i  is a detectable group; 
 A i  is a direct bond or a linker; 
 X i  is absent, an amino acid, or a peptide; and 
 R 1 ′ is an aspartic acid side-chain, an ester of aspartic acid, an aza-peptide epoxide modification of the aspartic acid, an aza-peptide Michael acceptor, an aldehyde modification of the aspartic terminal carboxyl group, a chloromethyl ketone group, or an acyloxy reactive group. 
 
     
     
         7 . The method of  claim 6  wherein the disease is a neurodegenerative disease or a cardiac disease. 
     
     
         8 . The method of  claim 7  wherein the disease is glaucoma, macular degeneration, proliferative retinopathy, neuropathy, Alzheimer's disease, multiple sclerosis, or Huntington's disease. 
     
     
         9 - 40 . (canceled) 
     
     
         41 . The method of  claim 6 , wherein detection is carried out using NMR, MRI, CT, CAT, PET scan, scintigraphy, a flow cytometer, a laser scanning cytometer, a fluorescence microplate reader, a luminescence microplate reader, a chromogenic microplate reader, a fluorescence microscope, a confocal microscope, a luminescence microscope, a bright-field microscope, a whole animal fluorescence imaging system, a whole animal luminescence imaging system, or a combination thereof. 
     
     
         42 . The method of  claim 6 , wherein detection is carried out using a window chamber that has been inserted into the animal. 
     
     
         43 . The method of  claim 6 , wherein detection is carried out using a fluorescence microscope, a confocal microscope, a bright-field microscope, or a luminescence microscope. 
     
     
         44 . The method of  claim 6 , wherein the caspase affinity labeling agent is introduced into the animal by intravenous, intravascular, intraperitoneal, intravitreal, intraocular, intracranial, intrapleural, intrathoracic, intramuscular, intrapulmonary, injection, perfusion, or lavage administration. 
     
     
         45 . The method of  claim 44 , wherein the caspase affinity labeling agent is introduced into the animal by intravenous administration. 
     
     
         46 . The method of  claim 6 , wherein the animal is a mammal. 
     
     
         47 . The method of  claim 48 , wherein the mammal is a human. 
     
     
         48 . The method of  claim 6 , wherein the detectable group comprises a Lanthanide series element. 
     
     
         49 . The method of  claim 6 , wherein the detectable group comprises a positron emitter. 
     
     
         50 . The method of  claim 6 , wherein the detectable group comprises a fluorescent label. 
     
     
         51 . The method of  claim 6 , wherein the detectable group comprises a radioisotope. 
     
     
         52 . The method of  claim 6 , wherein the detectable group is Gd, Tb, Eu, Ce, Pr, Nd, Pm, Sm, Dy, Ho, Er, Tm, Yb, Lu, Fe, Mn, Re, and Tc, I, Ba,  11 C,  13 N,  15 O,  64 Cu, a fluorescein, a sulforhodamine, a Cy dye, a BODIPY, a coumarin,  3 H,  14 C, or  35 S. 
     
     
         53 . The method of  claim 6 , wherein X 1  is the amino acid or amino acid sequence V, VA, YVA, DEV, LEE, LEH, VDVA (SEQ ID NO:1), IET, WHE, AEV, A, V, or E. 
     
     
         54 . The method of  claim 6 , wherein R 1 ′ is CH 2 —COOH, or —CH 2 CO 2 R, wherein R is C 1 -C 6  alkyl or benzyl, CH 3 , C 2 H 5 , or CH 2 C 6 H 5 . 
     
     
         55 . The method of  claim 6 , wherein the caspase affinity labeling agent is carboxyfluorescein-valanyl-alanyl-aspartyl(O-methyl)-fluoromethyl ketone. 
     
     
         56 . The method of  claim 6 , wherein the caspase affinity labeling agent binds covalently to the active catalytic site of a caspase and is retained within the cell.