US2015104418A1PendingUtilityA1

Bacterial composition

64
Assignee: MICROBIOS INCPriority: Dec 18, 2014Filed: Dec 18, 2014Published: Apr 16, 2015
Est. expiryDec 18, 2034(~8.4 yrs left)· nominal 20-yr term from priority
Inventors:Joseph F. FlintMatthew Ryan Garner
A61K 35/742A23Y 2220/07A23K 1/009A61K 35/744A23Y 2280/15A23K 10/18A23K 50/10A23K 50/20A61K 35/747A23K 50/75A23K 50/30A23V 2400/117A23V 2400/413
64
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Claims

Abstract

A bacterial composition that inhibits E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, together with commensurate inhibition rates against the Big-Six Escherichia coli strains referred to as the non-O157 STECs that include E. coli O121:H19; E. coli O45:H2; E. coli O103:H11; E. coli O145, E. coli O26:H11; and E. coli O111. The composition is constituted of various combinations of the following four unique pathogen-inhibiting bacteria: (1) Lactobacillus animalis strain MB101; (2) Lactobacillus animalis strain MB102; (3) Enterococcus faecium strain MB505; and (4) Pediococcus acidilactici strain MB902. Each of the discovered bacteria is deposited with the American Type Culture Collection (ATCC) and respectively has an individual ATCC Accession Number.

Claims

exact text as granted — not AI-modified
1 - 26 . (canceled) 
     
     
         27 . A method for preparing a probiotic composition comprising:
 mixing into a combination at least two bacteria selected from the group consisting of:
   Lactobacillus animalis  strain MB101 having ATCC Accession Number PTA-121710; 
   Lactobacillus animalis  strain MB102 having ATCC Accession Number PTA-121711; 
   Enterococcus faecium  strain MB505 having ATCC Accession Number PTA-121709; and 
   Pediococcus acidilactici  strain MB902 having ATCC Accession Number PTA-121712; and packaging the combination. 
   
     
     
         28 . The method recited in  claim 27  comprising mixing:
   Lactobacillus animalis  strain MB101 having ATCC Accession Number PTA-121710; and 
   Lactobacillus animalis  strain MB102 having ATCC Accession Number PTA-121711. 
 
     
     
         29 . The method recited in  claim 27  comprising mixing:
   Lactobacillus animalis  strain MB101 having ATCC Accession Number PTA-121710; 
   Lactobacillus animalis  strain MB102 having ATCC Accession Number PTA-121711; and 
   Enterococcus faecium  strain MB505 having ATCC Accession Number PTA-121709. 
 
     
     
         30 . The method recited in  claim 27  comprising mixing:
   Lactobacillus animalis  strain MB101 having ATCC Accession Number PTA-121710; 
   Lactobacillus animalis  strain MB102 having ATCC Accession Number PTA-121711; 
   Enterococcus faecium  strain MB505 having ATCC Accession Number PTA-121709; and 
   Pediococcus acidilactici  strain MB902 having ATCC Accession Number PTA-121712. 
 
     
     
         31 . The method recited in  claim 27  comprising mixing:
   Lactobacillus animalis  strain MB101 having ATCC Accession Number PTA-121710; and 
   Enterococcus faecium  strain MB505 having ATCC Accession Number PTA-121709. 
 
     
     
         32 . The method recited in  claim 27  comprising mixing:
   Lactobacillus animalis  strain MB101 having ATCC Accession Number PTA-121710; 
   Enterococcus faecium  strain MB505 having ATCC Accession Number PTA-121709; and 
   Pediococcus acidilactici  strain MB902 having ATCC Accession Number PTA-121712. 
 
     
     
         33 . The method recited in  claim 27  comprising mixing:
   Lactobacillus animalis  strain MB101 having ATCC Accession Number PTA-121710; and 
   Pediococcus acidilactici  strain MB902 having ATCC Accession Number PTA-121712. 
 
     
     
         34 . The method recited in  claim 27  comprising mixing:
   Lactobacillus animalis  strain MB101 having ATCC Accession Number PTA-121710; 
   Lactobacillus animalis  strain MB102 having ATCC Accession Number PTA-121711; and 
   Pediococcus acidilactici  strain MB902 having ATCC Accession Number PTA-121712. 
 
     
     
         35 . The method recited in  claim 27  comprising mixing:
   Lactobacillus animalis  strain MB102 having ATCC Accession Number PTA-121711; and 
   Enterococcus faecium  strain MB505 having ATCC Accession Number PTA-121709. 
 
     
     
         36 . The method recited in  claim 27  comprising mixing:
   Lactobacillus animalis  strain MB102 having ATCC Accession Number PTA-121711; 
   Enterococcus faecium  strain MB505 having ATCC Accession Number PTA-121709; and 
   Pediococcus acidilactici  strain MB902 having ATCC Accession Number PTA-121712. 
 
     
     
         37 . The method recited in  claim 27  comprising mixing:
   Lactobacillus animalis  strain MB102 having ATCC Accession Number PTA-121711; and 
   Pediococcus acidilactici  strain MB902 having ATCC Accession Number PTA-121712. 
 
     
     
         38 . The method recited in  claim 27  comprising mixing:
   Enterococcus faecium  strain MB505 having ATCC Accession Number PTA-121709; and 
   Pediococcus acidilactici  strain MB902 having ATCC Accession Number PTA-121712. 
 
     
     
         39 . The method recited in  claim 27  wherein at least one strain is a pathogen growth inhibitor. 
     
     
         40 . The method recited in  claim 27  wherein at least one strain is an  E. coli  O157:H7 growth inhibitor. 
     
     
         41 . The method recited in  claim 27  wherein at least one strain is a growth inhibitor to the non-O157 STEC, Big-Six  Escherichia coli  strains including  E. coli  O121:H19;  E. coli  O45:H2;  E. coli  O103:H11;  E. coli  O145,  E. coli  O26:H11; and  E. coli  O111. 
     
     
         42 . The method recited in  claim 27  wherein at least one strain is a  Salmonella  growth inhibitor. 
     
     
         43 . The method recited in  claim 27  wherein at least one strain is a  Salmonella typhimurium  growth inhibitor. 
     
     
         44 . The method recited in  claim 27  wherein at least one strain is a  Salmonella enteriditis  growth inhibitor. 
     
     
         45 . The method recited in  claim 27  wherein each strain is a pathogen growth inhibitor. 
     
     
         46 . The method recited in  claim 27  wherein each strain is an  E. coli  O157:H7 growth inhibitor. 
     
     
         47 . The method recited in  claim 27  wherein each strain is a growth inhibitor to the non-O157 STEC, Big-Six  Escherichia coli  strains including  E. coli  O121:H19;  E. coli  O45:H2;  E. coli  O103:H11;  E. coli  O145,  E. coli  O26:H11; and  E. coli  O111. 
     
     
         48 . The method recited in  claim 27  wherein each strain is a  Salmonella  growth inhibitor. 
     
     
         49 . The method recited in  claim 27  wherein each strain is a  Salmonella typhimurium  growth inhibitor. 
     
     
         50 . The method recited in  claim 27  wherein at least one strain is a  Salmonella enteriditis  growth inhibitor. 
     
     
         51 . The method recited in  claim 27  further comprises mixing in a carrier and thereby establishing a direct fed bacterial animal feed additive. 
     
     
         52 . A method for preparing a probiotic composition comprising:
 obtaining at least one bacteria selected from the group consisting of:
   Lactobacillus animalis  strain MB102 having ATCC Accession Number PTA-121711; 
   Enterococcus faecium  strain MB505 having ATCC Accession Number PTA-121709; and 
   Pediococcus acidilactici  strain MB902 having ATCC Accession Number PTA-121712; and 
   packaging the composition.

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