US2015110755A1PendingUtilityA1

Elastin producing fibroblast formulations and methods of using the same

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Assignee: HUMAN MATRIX SCIENCES LLCPriority: May 17, 2005Filed: Oct 25, 2014Published: Apr 23, 2015
Est. expiryMay 17, 2025(expired)· nominal 20-yr term from priority
Inventors:Felipe JimenezThomas MittsAleksander Hinek
A61P 43/00A61P 17/00A61K 31/353A61K 35/36A61Q 19/00A61K 31/366A61K 2800/91A61K 31/7048A61K 38/08A61K 8/985A61P 17/02A61Q 19/08A61K 35/33
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Claims

Abstract

The present invention describes therapeutic compositions comprising fibroblasts that have been stimulated to increase expression of extracellular matrix components or elastin, or to produce enhanced elastogenesis or the appearance thereof at a site of administration. The therapeutic fibroblast formulations can be prepared using a variety of elastogenic agents, including digests of mammalian elastin, chemically digested plant extracts comprising elastin-like peptides, and synthetic elastogenic peptides. The invention further comprises cosmetic and pharmaceutical treatment methods using the therapeutic fibroblast compositions of the invention.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . A therapeutic composition for stimulating elastogenesis or the appearance thereof comprising a therapeutically effective amount of a population of cultured dermal fibroblasts, wherein said population of cultured dermal fibroblasts is pretreated with an elastogenic composition. 
     
     
         2 . The therapeutic composition of  claim 1  wherein said population of cultured dermal fibroblasts is derived from a skin biopsy. 
     
     
         3 . The therapeutic composition of  claim 2  wherein said skin biopsy comprises cells of a stratum basale layer. 
     
     
         4 . The therapeutic composition of  claim 1 , wherein said elastogenic composition comprises at least one of ProK-60, E91, or ProK-60P. 
     
     
         5 . The therapeutic composition of  claim 1  wherein said elastogenic composition comprises a peptide having a sequence GXXPG (SEQ ID NO. 2), wherein X represents any of the natural amino acids. 
     
     
         6 . The therapeutic composition of  claim 1  wherein said elastogenic composition comprises a peptide having a sequence PGGVLPG (SEQ ID NO. 47), VGVVPG (SEQ ID NO. 48), or IGLGPGGV (SEQ ID NO. 49). 
     
     
         7 . The therapeutic composition of  claim 1  wherein said elastogenic composition comprises a peptide having a sequence VGAMPG (SEQ ID NO. 51), VGLSPG (SEQ ID NO. 52), IGAMPG (SEQ ID NO. 54), IGVAPG (SEQ ID NO. 55), IGLSPG (SEQ ID NO. 56), VGAMPGAAAAAVGAMPG (SEQ ID NO. 58), VGLSPGAAAAAVGLSPG (SEQ ID NO. 59), VGVAPGAAAAAVGVAPG (SEQ ID NO 60) IGAMPGAAAAAIGAMPG (SEQ ID NO. 61), IGLSPGAAAAAIGLSPG (SEQ ID NO. 63), or IGVAPGAAAAAIGVAPG (SEQ ID NO. 62). 
     
     
         8 . The therapeutic composition of  claim 1  wherein said elastogenic composition comprises a compound obtained or derived from a plant. 
     
     
         9 . The therapeutic composition of  claim 1  wherein said elastogenic composition comprises a compound obtained or derived from rice bran. 
     
     
         10 . A method for improving appearance or elasticity of a tissue comprising: administering to a mammal in need thereof an effective amount of a composition comprising a population of cultured dermal fibroblasts, wherein said population of cultured dermal fibroblasts is pretreated with an elastogenic composition. 
     
     
         11 . The method of  claim 10 , wherein said elastogenic composition comprises a compound selected from ProK-60, E91, and ProK-60P. 
     
     
         12 . The method of  claim 10  wherein said elastogenic composition comprises a peptide having a sequence GXXPG (SEQ ID NO. 2), wherein X represents any of the natural amino acids. 
     
     
         13 . The method of  claim 10  wherein said elastogenic composition comprises a peptide having a sequence PGGVLPG (SEQ ID NO. 47), VGVVPG (SEQ ID NO. 48), or IGLGPGGV (SEQ ID NO. 49). 
     
     
         14 . The method of  claim 10 , wherein said elastogenic composition comprises a peptide having a sequence VGAMPG (SEQ ID NO. 51), VGLSPG (SEQ ID NO. 52), IGAMPG (SEQ ID NO. 54), IGVAPG (SEQ ID NO. 55), IGLSPG (SEQ ID NO. 56), VGAMPGAAAAAVGAMPG (SEQ ID NO. 58), VGLSPGAAAAAVGLSPG (SEQ ID NO. 59), VGVAPGAAAAAVGVAPG (SEQ ID NO 60) IGAMPGAAAAAIGAMPG (SEQ ID NO. 61), IGLSPGAAAAAIGLSPG (SEQ ID NO. 63), or IGVAPGAAAAAIGVAPG (SEQ ID NO. 62). 
     
     
         15 . The method of  claim 10  wherein said elastogenic composition is obtained or derived from a plant. 
     
     
         16 . The method of  claim 10  wherein said elastogenic composition is obtained or derived from rice bran. 
     
     
         17 . The method of  claim 10 , wherein said composition is administered by injection. 
     
     
         18 . The method of  claim 10 , wherein said composition stimulates elastogenesis at the site of administration. 
     
     
         19 . The method of  claim 10 , wherein said composition improves the appearance of visible lines or wrinkles. 
     
     
         20 . The method of  claim 10 , wherein said composition improves the appearance of scar tissue. 
     
     
         21 . A method for stimulating new blood vessel formation in a tissue comprising: administering to a mammal in need thereof an effective amount of a composition comprising a population of cultured dermal fibroblasts, wherein said population of cultured dermal fibroblasts is pretreated with an elastin digest preparation. 
     
     
         22 . The method of  claim 21  wherein said administration is by injection. 
     
     
         23 . The method of  claim 21  wherein said administration is performed in preparation for plastic surgery.

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