Tnf-alpha binding proteins

Abstract

TNF-α binding proteins, including chimeric, CDR-grafted, and humanized antibodies that bind TNF-α are provided. Binding proteins have high affinity for TNF-α and neutralize TNF-α activity. A binding protein can be a full-length antibody or a TNF-α-binding portion thereof. Methods of making and methods of using the binding proteins are also described. The TNF-α binding proteins are useful for detecting TNF-α and for inhibiting TNF-α activity, including in a human subject suffering from a disease or disorder in which TNF-α activity is detrimental.

1 - 29 . (canceled) 
     
     
         30 . An isolated nucleic acid encoding a binding protein or antigen binding portion thereof amino acid sequence of SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, or SEQ ID NO: 46. 
     
     
         31 . A vector comprising the isolated nucleic acid of  claim 30 . 
     
     
         32 . (canceled) 
     
     
         32 . A host cell comprising the vector of  claim 31 . 
     
     
         33 . The host cell of  claim 32 , wherein said host cell is a prokaryotic cell. 
     
     
         34 . The host cell of  32 , wherein said host cell is a eukaryotic cell. 
     
     
         35 . The host cell of  claim 34 , wherein said eukaryotic cell is a protist cell, an animal cell, a plant cell, a fungal cell, a mammalian cell, an avian cell, or an insect cell. 
     
     
         36 . The host cell of  claim 35 , wherein said eukaryotic cell is  S. cerevisiae , CHO cell, COS cell, or SF9 cell. 
     
     
         37 . A method of producing a protein capable of binding TNF-α, the method comprising the steps of culturing the host cell of  claim 32  in culture medium under conditions sufficient to produce a binding protein capable of binding TNF-α. 
     
     
         38 - 53 . (canceled) 
     
     
         54 . The vector of  claim 31 , wherein said vector is pcDNA, pTT, pTT3, pEFBOS, pBV, pJV, pHybE, or pBJ.