Method for Making High Maltose Syrup

Abstract

The present teachings provide direct conversion of granular starch into a soluble sugar composition comprising a high, very high, and/or ultra high maltose content. The method involves contacting an aqueous slurry of granular starch with an enzyme composition comprising an appropriate ratio of an alpha-amylase and a maltogenic enzyme to produce a soluble starch substrate that is enzymatically converted to a sugar composition containing higher maltose. The process may use more than one temperature to achieve the same.

1 . A method of making a high DP2 syrup containing at least 50% DP2 comprising;
 solubilizing a granular starch substrate at or below the initial gelatinization temperature with an exogenous alpha-amylase to form a mixture comprising dextrins;   hydrolyzing the dextrins with a maltogenic enzyme to form the high DP2 syrup, wherein the ratio of alpha-amylase dose expressed as AAU/gds, to maltogenic enzyme dose expressed as DP degrees, is less than 8.   
     
     
         2 - 3 . (canceled) 
     
     
         4 . The method according to  claim 1  further comprising a debranching enzyme 
     
     
         5 . (canceled) 
     
     
         6 . The method according to  claim 1  wherein the starch is whole ground grain selected from the group consisting of corn, wheat, barley, rye, triticale, rice, oat, beans, banana, potato, sweet potato, sorghum, legumes, cassava, millet, potato, or tapioca. 
     
     
         7 - 12 . (canceled) 
     
     
         13 . The method according to  claim 1  wherein the maltogenic enzyme is selected from the group consisting of a barley beta-amylase OPTIMALT® BBA or Betalase 1500, a wheat beta-amylase, a soy beta-amylase β-amylase#1500S, a fungal alpha-amylase CLARASE® L, maltogenic amylase is MAX-LIFE™ P100 or Maltogenase L. 
     
     
         14 - 19 . (canceled) 
     
     
         20 . The method according to  claim 1  wherein the maltogenic enzyme is Fungamyl 800L, Novamyl ( Bacillus  sp.), GC 626 (AKAA), Veron® XTENDER, beta-amylases from plant material (pea, sweet potato, rye, oats, rice, sorghum), a microbial beta-amylase ( paenibacillus polymyxa, Thermoanaerobacterium thermosulfurigenes, Xanthophyllomyces dendrorhous, Bacillus cereus, Bacillus megaterium, Arabidopsis thaliana ), a fungal alpha-amylase from  Aspergillus  (e.g.,  A. Niger, A. kawachi , and  A. oryzae ),  Trichoderma  sp. (e.g.  Trichoderma reesei  alpha-amylase),  Rhisopus  sp.,  Mucor  sp., and  Penicillium  sp., or a maltogenic amylase from  Bacillus  sp.,  Bacillus subtilis, Geobacillus stearothermophilus, Lactobacillus gasseri  and  Thermus  sp. 
     
     
         21 . The method according to  claim 1  wherein the alpha-amylase is selected from the group consisting of SPEZYME® XTRA, SPEZYME® FRED, SPEZYME® LT 300, BAN® 480L, Liquozyme® Supra, Liquozyme® SCDS, MAX-LIFE™ P100, Maltogenase L, CLARASE® L, Liquozyme® SC, Termamyl® SC, Fuelzyme® LF, Veretase, Liqozyme® SC4x, Liquozyme® Supra 2.8, Liquozyme® supra 2, Liquozyme® X, Termamyl® 120L, SPEZYME® ALPHA, SPEZYME® CL, Clearflow® AA, Optitherm™ and Takatherm™, Keistase™, an alpha-amylase from  Bacillus  species including  B. subtilis, B. stearothermophilus, B. lentus, B. licheniformis, B. coagulans , and  B. amyloliquefaciens , fungal alpha-amylases from  Aspergillus  (e.g.,  A. Niger, A. kawachi , and  A. oryzae ),  Trichoderma  sp. (e.g.  Trichoderma reesei  alpha-amylase),  Rhisopus  sp.,  Mucor  sp., and  Penicillium  sp., or a maltogenic amylases from  Bacillus  sp.,  Bacillus subtilis, Geobacillus stearothermophilus, Lactobacillus gasseri  and  Thermus  sp. 
     
     
         22 . (canceled) 
     
     
         23 . The method according to  claim 1  wherein the debranching enzyme, if present, is selected from the group consisting of OPTIMAX® L-1000, Promozyme® D2, or ISOAMYLASE from  Pseudomonas  sp. (e.g. Megazyme), Promozyme D6 (Novozymes), pullulanases secreted by a  Bacillus  species such as  Bacillus deramificans, Bacillus acidopullulyticus , and  Bacillus naganoensis , pullulanase from  Sulfolobus solfataricus, Pseudomonas  sp. and thermostable pullulanase from  Fervidobacterium nodosum.    
     
     
         24 . (canceled) 
     
     
         25 . A composition comprising an alpha-amylase and a maltogenic enzyme wherein the ratio of alpha-amylase dose expressed as AAU/gds, to maltogenic enzyme dose expressed as DP degrees, is 0.002-7.94. 
     
     
         26 . (canceled) 
     
     
         27 . The composition according to  claim 25  further comprising a pullulanase. 
     
     
         28 . The composition according to  claim 25  further comprising granular starch. 
     
     
         29 - 31 . (canceled) 
     
     
         32 . A method of making high DP2 syrup containing at least 50% DP2 comprising; solubilizing a granular starch substrate below the initial gelatinization temperature with an exogenous alpha-amylase and exogenous beta amylase; and
 hydrolyzing the resulting mixture at a higher temperature than the first temperature, both of which are below the initial gelatinization temperature, to form the high DP2 syrup.   
     
     
         33 . A method according to  claim 32  wherein the first temperature is kept for 1 to 3 hours. 
     
     
         34 . A method according to  claim 32  wherein the first temperature is about 50° C. 
     
     
         35 . A method according to  claim 32  wherein the second temperature is about 55° C. to about 60° C. 
     
     
         36 - 39 . (canceled) 
     
     
         40 . A method according to  claim 32  wherein the peak viscosity is reduced by at least about 10% compared to the process when done at the higher temperature only. 
     
     
         41 . A method according to  claim 32  wherein the filtration speed is improved by at least about 1.5-fold compared to the process when done at the higher temperature only. 
     
     
         42 - 43 . (canceled)