System and method including analytical units

Abstract

Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.

1 . A cartridge loading unit for loading assay cartridges onto an automated system, comprising:
 a presentation lane including a carriage to receive an assay cartridge, the presentation lane configured to transport the assay cartridge into the automated system for processing; and   a first loading lane including a cavity to receive a DNA assay cartridge and transfer the DNA assay cartridge to the carriage of the presentation lane, wherein the DNA assay cartridge includes a reaction well and a reagent compartment, the reagent compartment containing a reagent used for DNA extraction from a sample.   
     
     
         2 . The cartridge loading unit of  claim 1  further including a second loading lane configured to transfer an RNA assay cartridge to the presentation lane, wherein the RNA assay cartridge includes a reaction well and an RNA reagent compartment, the RNA reagent compartment containing a reagent used for RNA extraction from the sample. 
     
     
         3 . The cartridge loading unit of  claim 1 , wherein the reaction well of the DNA assay cartridge is asymmetrical and includes an endwall having a tapered segment, the tapered segment having a radius profile to create a frusto-conical surfaced culvert directed to the bottom of the reaction well. 
     
     
         4 . The cartridge loading unit of  claim 1 , wherein the reagent used for DNA extraction from a sample is one of a wash buffer, an elution buffer, a magnetically responsive microparticle, and a binding buffer 
     
     
         5 . The cartridge loading unit of  claim 2 , wherein the reagent used for RNA extraction from the sample is one of a wash buffer, an elution buffer, a magnetically responsive particle, and a binding buffer. 
     
     
         6 . The cartridge loading unit of  claim 4  further including a second loading lane configured to transfer an RNA assay cartridge to the presentation lane, wherein the RNA assay cartridge includes a reaction well and an RNA reagent compartment, the RNA reagent compartment containing a reagent used for RNA extraction from a second sample. 
     
     
         7 . The cartridge loading unit of  claim 1 , wherein the DNA assay cartridge further includes a pipette tip and a reaction vessel. 
     
     
         8 . The cartridge loading unit of  claim 7 , wherein the reaction vessel is configured to receive the extracted DNA from the sample and an amplification reagent. 
     
     
         9 . The cartridge loading unit of  claim 8 , wherein the amplification reagent is not contained on the DNA assay cartridge. 
     
     
         10 . The cartridge loading unit of  claim 1  further including an identification bar, the DNA assay cartridge further including a keying feature, wherein the identification bar is arranged in the first loading lane to correspond with the keying feature of the DNA assay cartridge to prevent loading of an RNA assay cartridge. 
     
     
         11 . The cartridge loading unit of  claim 1 , wherein the first loading lane includes a pusher that impels the DNA assay cartridge from the first loading lane to the presentation lane. 
     
     
         12 . The cartridge loading unit of  claim 1  wherein the presentation lane comprises a presentation rail. 
     
     
         13 . The cartridge loading unit of  claim 12  wherein the presentation lane is oriented perpendicular to the first loading lane. 
     
     
         14 . The cartridge loading unit of  claim 1  wherein the presentation lane is oriented perpendicular to the first loading lane. 
     
     
         15 . The cartridge loading unit of  claim 1  wherein the presentation lane further comprises a presentation guide. 
     
     
         16 . The cartridge loading unit of  claim 15  wherein the presentation guide comprises a gap configured to allow a pipettor to access the DNA assay cartridge when the DNA assay cartridge is in the presentation lane. 
     
     
         17 . The cartridge loading unit of  claim 1  wherein the first loading lane is a first onload lane. 
     
     
         18 . The cartridge loading unit of  claim 2  wherein the first and second loading lanes are first and second onload lanes, respectively.