Column packing material, column using the same, and method of separation using the same

Abstract

Aimed at readily and exactly separate 8-hydroxy-2′-deoxyguanosine (8-OHdG) contained in a sample, a column packing material used for separating 8-OHdG, which contains a packing material composed of a material having a straight-chain hydrocarbon group having 6 or more and 30 or less carbon atoms as a functional group, and having a carbon content over the surface of carrier of 18% or less by element ratio, wherein the packing material contains 1 cumulative percent or more and 20 cumulative percent or less, on the particle-count basis, of particles having a circle-equivalent diameter, measured using a flow-type particle image analyzer, of 0.5 μm or larger and 10 μm or smaller, is used.

1 - 14 . (canceled) 
     
     
         15 . A column packing material used for separating 8-hydroxy-2′-deoxyguanosine (8-OHdG),
 containing a packing material composed of a material having a straight-chain hydrocarbon group having 6 or more and 30 or less carbon atoms as a functional group, and having a carbon content over the surface of carrier of 18% or less by element ratio, 
 wherein said packing material contains 1 cumulative percent or more and 20 cumulative percent or less, on the particle-count basis, of particles having a circle-equivalent diameter, measured using a flow-type particle image analyzer, of 0.5 μm or larger and 10 μm or smaller, 
 wherein said packing material contains particles having a circle-equivalent diameter, measured using a flow-type particle image analyzer, of 35 μm or larger 60 μm or smaller, and particles having a circle-equivalent diameter of 10 μm or larger and 35 μm or smaller, at a ratio by weight of 80:20 to 95:5. 
 
     
     
         16 . The column packing material used for separating 8-hydroxy-2′-deoxyguanosine (8-OHdG) as claimed in  claim 15 ,
 further containing 65 cumulative percent or more and 99 cumulative percent or less, on the particle-count basis, of particles having a circle-equivalent diameter, measured using a flow-type particle image analyzer, of 20 μm or larger and 100 μm or smaller. 
 
     
     
         17 . The column packing material used for separating 8-hydroxy-2′-deoxyguanosine (8-OHdG) as claimed in  claim 15 ,
 further containing 78 cumulative percent or more and 99 cumulative percent or less, on the particle-count basis, of particles having a circle-equivalent diameter, measured using a flow-type particle image analyzer, of 20 μm or larger and 100 μm or smaller. 
 
     
     
         18 . The column packing material as claimed in  claim 15 ,
 wherein said material composing said packing material is a silica gel having octadecyl group.   
     
     
         19 . The column packing material as claimed in  claim 16 ,
 wherein said material composing said packing material is a silica gel having octadecyl group.   
     
     
         20 . The column packing material as claimed in  claim 17 ,
 wherein said material composing said packing material is a silica gel having octadecyl group.