Fc VARIANTS WITH ALTERED BINDING TO FcRn

Abstract

The present application relates to optimized IgG immunoglobulin variants, engineering methods for their generation, and their application, particularly for therapeutic purposes.

1 - 24 . (canceled) 
     
     
         25 . A polypeptide comprising a variant Fc region as compared to a parent Fc region, said variant Fc region comprising amino acid substitutions at position 434, wherein said amino acid substitution is N434S, and wherein numbering is according to the EU Index in Kabat et al. 
     
     
         26 . A polypeptide according to  claim 25 , wherein said polypeptide is a member selected from the group consisting of an antibody and an Fc fusion protein. 
     
     
         27 . A polypeptide according to  claim 26 , wherein said polypeptide is an antibody. 
     
     
         28 . A polypeptide according to  claim 27 , wherein said antibody is selected from the group consisting of a chimeric antibody, a humanized antibody, a monoclonal antibody, and a human antibody. 
     
     
         29 . A polypeptide according to  claim 26 , wherein said polypeptide is an Fc fusion protein. 
     
     
         30 . A method of producing a polypeptide according to  claim 25 , said method comprising providing a cell comprising a nucleic acid encoding said polypeptide, wherein said cell is cultured under conditions suitable for expression of said polypeptide. 
     
     
         31 . A method according to  claim 30 , wherein said nucleic acid is contained in an expression vector. 
     
     
         32 . A host cell comprising a nucleic acid encoding a polypeptide according to  claim 25 . 
     
     
         33 . An expression vector, wherein said expression vector encodes a polypeptide according to  claim 25 . 
     
     
         34 . An anti-C5 antibody comprising a variant Fc region as compared to a parent IgG Fc polypeptide, wherein said variant Fc region comprises amino acid substitutions at position 434, wherein said amino acid substitution is N434S, and wherein numbering is according to the EU Index in Kabat et al.