Systems and methods for isolating and using clinically safe adipose derived regenerative cells

Abstract

Systems and methods are described that are used to separate cells from a wide variety of tissues. In particular, automated systems and methods are described that separate regenerative cells, e.g., stem and/or progenitor cells, from adipose tissue. The systems and methods described herein provide rapid and reliable methods of separating and concentrating regenerative cells suitable for re-infusion into a subject.

What is claimed is: 
     
         1 . A tissue removal system for treating a patient, comprising:
 (a) a tissue collection container including:
 (i) a tissue collecting inlet port structured to receive adipose tissue removed from a patient; and 
 (ii) a filter disposed within the container and being structured to retain adipose tissue removed from a patient and to pass non-adipose tissue removed from the patient; 
   (b) a mixing container coupled to the tissue collection container to receive stem cells obtained from the adipose tissue without removal of the stem cells from the tissue removal system, and including an additive port for the administration of at least one additive to mix with the stem cells contained therein; and   (c) an outlet structured to permit the stem cells in the mixing container to be removed from the tissue collection system for administration to a patient.