Integrated type microfluidic electrochemical biosensor system and method for rapid biochemical analysis

Abstract

The present invention provides an integrated type microfluidic electrochemical biosensor system for rapid biochemical analysis and the usage of the system. The system comprising: a continuous feeding unit for sequentially conveying lead eluent, sample solution, sample eluent, signal probe solution, signal probe eluent and electrochemical detection buffer solution; a microfluidic chip consists of one or more micro-channel network, the microfluidic chip covers the electrode array to form a channel system, capture probes which have interaction with the said sample solution fixed on the surface of the electrode array, said channel system is connected with the continuous feed unit; and a power system for providing power to said continuous feeding unit. The invention innovatively combine three technologies of planar electrode arrays, microfluidic chip technology and continuous feeding unit together, and the integrated type microfluidic electrochemical biosensing system which is small in size and low in cost and has a wide application prospect is provided.

1 . An integrated type type microfluidic electrochemical biosensor system for rapid biochemical analysis, characterized by comprising:
 a continuous feeding unit for sequentially conveying lead eluent, sample solution, sample eluent, signal probe solution, signal probe eluent and electrochemical detection buffer solution;   a microfluidic chip consists of one or more micro-channel network, the microfluidic chip covers the electrode array to form a channel system, capture probes which have interaction with the said sample solution fixed on the surface of the electrode array, said channel system is connected with the continuous feed unit; and   a power system for providing power to said continuous feeding unit.   
     
     
         2 . The microfluidic electrochemical biosensor system according to  claim 1 , wherein
 The microfluidic chip and electrode array formed no-leakage reversible or irreversible channel system by plasma cleaning and heat bonding process.   
     
     
         3 . The microfluidic electrochemical biosensor system according to  claim 2 , wherein
 the condition of the heat bonding process is heated above 37° C. for more than 30 minutes.   
     
     
         4 . The microfluidic electrochemical biosensor system according to  claim 1 , wherein
 the electrode array is a carbon electrode array or gold electrode array prepared by the screen printing technique, or an electrode array produced by directly depositing nano metal particles on the surface of the carbon electrode electrochemical deposition of the electrode array or the nano metal, or a planar electrode array produced by photolithography technique.   
     
     
         5 . The microfluidic electrochemical biosensor system according to  claim 1 , wherein
 the capture probes fixed on the surface of the electrode array include antibodies, antigens, nucleic acids or nucleic acid aptamers.   
     
     
         6 . The microfluidic electrochemical biosensor system according to  claim 1 , wherein
 the continuous feeding unit is consists of small tube which have through channel, and said lead eluent, sample solution, sample eluent, signal probe solution, signal probe eluent and electrochemical detection buffer solution spaced by the air bubble continuously went through the through channel into the micro-channel network of said microfluidic chip.   
     
     
         7 . The microfluidic electrochemical biosensor system according to  claim 1 , wherein
 The power system is consists of the injection pump or injector connecting the downstream of the microfluidic chip and provide vacuum negative pressure as the fluid driving force to achieve the automatic transfer of solutions with different functions of within the continuous feeding unit.   
     
     
         8 . An usage of integrated type microfluidic electrochemical biosensor system for rapid biochemical analysis, which is characterized by comprising:
 providing an integrated type microfluidic electrochemical biosensor system according to  claim 1 ;   connecting the power system to the downstream of the microfluidic chip, sequentially conveying lead eluent, sample solution, sample eluent, signal probe solution, signal probe eluent and electrochemical detection buffer solution into said microfluidic chip's micro-channel network by said continuous feeding unit and interacting with the capture probe fixed on the surface of the electrode array, achieving continuous capture and cleaning, generating a detectable electrochemical signal quickly, reading out electrochemical signal of the electrode array surface modified by different capture probes once by electrochemical workstation.   
     
     
         9 . The usage according to  claim 8 , wherein
 the lead eluent, the sample solution, the sample eluate, the signal probe solution, signal probe elution, and the electrochemical detection buffer solution sequentially spaced by air bubbles with length of 0.5 cm or more to prevent cross-contamination.   
     
     
         10 . The usage according to  claim 9 , wherein
 the power system is consists of the injection pump or injector connecting the downstream of the microfluidic chip, and said injection pump or injector connect to the outlet end of said microfluidic chip by a period of small rubber tube which have strong deformability to form a vacuum negative pressure system.