Preservation Solutions For Biologics And Methods Related Thereto

Abstract

The present invention provides compositions (i.e., preservation solutions) for preserving biologics comprising at least one membrane penetrable sugar, at least one membrane impenetrable sugar, at least one anti-microbial agent, at least one anti-oxidant, adenosine albutinum, a salt, a buffer, and a chelating agent, and to methods of using such preservation solutions to preserve biologies.

1 - 17 . (canceled) 
     
     
         18 . A method of preserving a cancer cell comprising:
 contacting the cancer cell with a solution, wherein the solution comprises:
 a buffer; 
 from about 0.01% to about 10% salt; 
 at least one membrane penetrable sugar; 
 a neutral dextran with a molecular weight greater than or equal to 50,000 daltons; and 
 0.1% w/v to about 12% w/v glucose; and 
   drying the cancer cell by room temperature induced dryness.   
     
     
         19 . The method of  claim 18 , wherein the buffer is from about 0.1 mg/mL, to about 2.0 mg/mL, phosphate buffer. 
     
     
         20 . The method of  claim 18 , wherein the buffer is 0.1 M HEPES. 
     
     
         21 . The method of  claim 18 , wherein the salt is from about 0.25% to about 2% NaCl. 
     
     
         22 . The method of  claim 18 , wherein the at least one membrane penetrable sugar is trehalose. 
     
     
         23 . The method of  claim 22 , wherein trehalose is 0.1% w/v to about 12% w/v. 
     
     
         24 . The method of  claim 18 , wherein the neutral dextran with a molecular weight greater than or equal to 50,000 daltons is Dextran-70. 
     
     
         25 . The method of  claim 24 , wherein the Dextran-70 is from about 0.01% w/v to about 25% w/v. 
     
     
         26 . The method of  claim 24 , wherein the Dextran-70 is 6% w/v. 
     
     
         27 . The method of  claim 18 , wherein the glucose is about 2% w/v. 
     
     
         28 . The method of  claim 18 , further comprising rehydrating the cancer cell. 
     
     
         29 . The method of  claim 18 , wherein:
 the buffer is 0.1 M HEPES;   the salt is from about 0.25% to about 2% NaCl;   the at least one membrane penetrable sugar is 0.1% w/v to about 12% w/v trehalose;   the neutral dextran with a molecular weight greater than or equal to 50,000 daltons is from about 0.01% w/v to about 25% w/v Dextran-70; and   the glucose is 0.1% w/v to about 12% w/v glucose.   
     
     
         30 . A method of preserving a cancer cell comprising:
 contacting the cancer cell with a solution, wherein the solution comprises:
 0.1 M HEPES; 
 from about 0.25% to about 2% NaCl; 
 0.1%) w/v to about 12%) w/v trehalose; 
 6% w/v Dextran-70; and 
 about 2% w/v glucose; and drying the cell at room temperature.